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KD/KO Validated

PKC Delta Polyclonal antibody

PKC Delta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Publications

Cat no : 19132-1-AP

Print datasheet

Synonyms

PRKCD, PKCD, nPKC-delta, MAY1, EC:2.7.11.13

验证数据展示

WB analysis using 19132-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC Delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 19132-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19132-1-AP (PKC delta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PRKCD antibody included
IHC staining of human brain using 19132-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19132-1-AP (PKC delta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with PRKCD antibody included
IP experiment of A431 using 19132-1-AP
IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:800) with A431 cells lysate 2640 ug.
IP experiment of A431 using 19132-1-AP
IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:500) with A431 cells lysate 2000ug.
IF Staining of U2OS using 19132-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 19132-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Delta (19132-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IF Staining of U-251 using 19132-1-AP
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 19132-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
WB analysis using 19132-1-AP
Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently.

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Cat No. 19132-1-AP

Size

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经过测试的应用

Positive WB detected in	A431 cells, Jurkat cells, MCF-7 cells, HT-1080 cells, mouse colon tissue, HUVEC cells
Positive IP detected in	A431 cells
Positive IHC detected in	human brain tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells, U-251 cells, HeLa cells, A431 cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy PRKCD Ready-To-Use IHC Kit. PRKCD primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

产品信息

19132-1-AP targets PKC Delta in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IP, CoIP, IF
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	PKC Delta fusion protein Ag5631 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	protein kinase C, delta
Synonyms	PRKCD, PKCD, nPKC-delta, MAY1, EC:2.7.11.13
Calculated Molecular Weight	78 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC043350
Gene Symbol	PKC Delta
Gene ID (NCBI)	5580
RRID	AB_10637263
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q05655
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
WB protocol for PKC Delta antibody 19132-1-AP	Download protocol
IHC protocol for PKC Delta antibody 19132-1-AP	Download protocol
IF protocol for PKC Delta antibody 19132-1-AP	Download protocol
IP protocol for PKC Delta antibody 19132-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IHC,IF,IP,CoIP	Oncogene TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD Authors - Linqing Sun View Article
mouse	WB	Virol Sin Rottlerin plays an antiviral role at early and late steps of Zika virus infection Authors - Shili Zhou KD Validated View Article
rat	WB	Food Chem Toxicol G-Rh4 improves pancreatic β-cells dysfunction in vivo and in vitro by increased expression of Nrf2 and its target genes. Authors - Yao Liu View Article
	WB	Fish Shellfish Immunol In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I. Authors - Zhong-Wen Chang View Article
mouse	WB	iScience Induction of Inflammatory Macrophages in the Gut and Extra-Gut Tissues by Colitis-Mediated Escherichia coli. Authors - Houbao Qi View Article
mouse	WB	Neurochem Int Protein kinase C-mediated impairment of glutamine outward transport and SN1 transporter distribution by ammonia in mouse cortical astrocytes. Authors - Katarzyna Dąbrowska View Article

PKC Delta Polyclonal antibody

PKC Delta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

验证数据展示

WB analysis using 19132-1-AP

IHC staining of human brain using 19132-1-AP

IHC staining of human brain using 19132-1-AP

IP experiment of A431 using 19132-1-AP

IP experiment of A431 using 19132-1-AP

IF Staining of U2OS using 19132-1-AP

FC experiment of HeLa using 19132-1-AP

IF Staining of U-251 using 19132-1-AP

IF Staining of HeLa using 19132-1-AP

WB analysis using 19132-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD

Rottlerin plays an antiviral role at early and late steps of Zika virus infection

G-Rh4 improves pancreatic β-cells dysfunction in vivo and in vitro by increased expression of Nrf2 and its target genes.

In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.

Induction of Inflammatory Macrophages in the Gut and Extra-Gut Tissues by Colitis-Mediated Escherichia coli.

Protein kinase C-mediated impairment of glutamine outward transport and SN1 transporter distribution by ammonia in mouse cortical astrocytes.

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Flow Cytometry Perm Buffer (10X)