验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, RAW 264.7 cells, HeLa cells, NIH/3T3 cells, HEK-293 cells, Raji cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
COX-2 expression is undetectable in most normal tissues but dramatically upregulated during inflammation (PMID: 9249646, 18657230).
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12375-1-AP targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Immunogen | COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag3025 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) |
| Synonyms | COX2, PTGS2, GRIPGHS, EC:1.14.99.1, Cyclooxygenase-2 |
| Calculated Molecular Weight | 604 aa, 68 kDa |
| Observed Molecular Weight | 70-74 kDa |
| GenBank Accession Number | BC013734 |
| Gene Symbol | COX2/PTGS2 |
| Gene ID (NCBI) | 5743 |
| RRID | AB_2085127 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35354 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COX2 is an enzyme involved in the synthesis of prostaglandins from arachidonic acid.
1. What is the molecular weight of COX2?
The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also
expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented
PTGS2 has also previously been detected in AD brains (PMID:14724276).
2. Is COX2 post-translationally modified?
COX2 is a subject of post-translational modifications, including phosphorylation, glycosylation, and
s-nitrosylation (PMID: 28939645).
3. What is the difference between COX1, COX2, and COX3?
There are three isoenzymes that have cyclooxygenase activity: COX1, COX2, and COX3. While COX1
is a ubiquitously expressed constitutive enzyme, expression of COX2 is generally low but can be rapidly
induced by various stimuli (as part of infection and inflammatory response) and is controlled by the
transcription factor NFκB. COX3 is an alternative splice variant of COX1 enzyme and is considered
non-functional in humans.
4. I cannot detect COX2 in my sample during western blotting.
Unlike COX1, COX2 expression is inducible by a variety of stimuli including certain growth factors, cytokines,
and proinflammatory stimuli. COX2 basal expression levels may be very low in unstimulated cells. Additionally,
the COX2 half-life time is short and after stimulation, COX2 protein can be quickly degraded to basal levels
(PMID: 10966456), which should be taken into account during experimental design.
5. What is the role of COX2 in cancer?
COX2 is often upregulated in various cancer types and increased expression of COX2 is associated with
greater angiogenesis of solid tumors, increased invasion, and metastasis, as well as decreased host immunity.
6. What is subcellular localization of COX2?
Both COX1 and COX2 are present in the endoplasmic reticulum (ER) and nuclear envelope (PMID: 9545330),
but COX2 has been reported to be more enriched in the nuclear envelope compared to COX1 (PMID: 7738031).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 12375-1-AP | Download protocol |
| IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 12375-1-AP | Download protocol |
| IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 12375-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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