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COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal antibody

COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12375-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (3)

应用

WB, IHC, IF/ICC, ELISA and More (2)

COX2, PTGS2, GRIPGHS, EC:1.14.99.1, Cyclooxygenase-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, RAW 264.7 cells, HeLa cells, NIH/3T3 cells, HEK-293 cells, Raji cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

COX-2 expression is undetectable in most normal tissues but dramatically upregulated during inflammation (PMID: 9249646, 18657230).

Planning an IHC experiment? We recommend our IHCeasy COX2/PTGS2 Ready-To-Use IHC Kit. COX2/PTGS2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12375-1-AP targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, pig, chicken
免疫原 COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag3025 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
别名 COX2, PTGS2, GRIPGHS, EC:1.14.99.1, Cyclooxygenase-2
计算分子量 604 aa, 68 kDa
观测分子量 70-74 kDa
GenBank蛋白编号BC013734
基因名称 COX2/PTGS2
Gene ID (NCBI) 5743
RRIDAB_2085127
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35354
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX2 is an enzyme involved in the synthesis of prostaglandins from arachidonic acid.


1. What is the molecular weight of COX2?

The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also 

expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented 

PTGS2 has also previously been detected in AD brains (PMID:14724276).

2. Is COX2 post-translationally modified?

COX2 is a subject of post-translational modifications, including phosphorylation, glycosylation, and 

s-nitrosylation (PMID: 28939645).

3. What is the difference between COX1, COX2, and COX3?

There are three isoenzymes that have cyclooxygenase activity: COX1, COX2, and COX3. While COX1 

is a ubiquitously expressed constitutive enzyme, expression of COX2 is generally low but can be rapidly 

induced by various stimuli (as part of infection and inflammatory response) and is controlled by the 

transcription factor NFκB. COX3 is an alternative splice variant of COX1 enzyme and is considered 

non-functional in humans. 

4. I cannot detect COX2 in my sample during western blotting.

Unlike COX1, COX2 expression is inducible by a variety of stimuli including certain growth factors, cytokines, 

and proinflammatory stimuli. COX2 basal expression levels may be very low in unstimulated cells. Additionally, 

the COX2 half-life time is short and after stimulation, COX2 protein can be quickly degraded to basal levels 

(PMID: 10966456), which should be taken into account during experimental design.

5. What is the role of COX2 in cancer?

COX2 is often upregulated in various cancer types and increased expression of COX2 is associated with 

greater angiogenesis of solid tumors, increased invasion, and metastasis, as well as decreased host immunity.

6. What is subcellular localization of COX2?

Both COX1 and COX2 are present in the endoplasmic reticulum (ER) and nuclear envelope (PMID: 9545330), 

but COX2 has been reported to be more enriched in the nuclear envelope compared to COX1 (PMID: 7738031).


实验方案

Product Specific Protocols
WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 12375-1-APDownload protocol
IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 12375-1-APDownload protocol
IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 12375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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