Phospho-AKT (Ser473) Recombinant antibody
Phospho-AKT (Ser473) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (5)
Applications
WB, FC (Intra), ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
2E17
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HEK-293T cells, HeLa cells, IGF-1 treated HEK-293T cells, Calyculin A treated HEK-293 cells, Calyculin A treated HeLa cells |
Positive FC (Intra) detected in | Calyculin A treated HEK-293 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80455-1-RR targets Phospho-AKT (Ser473) in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | WB, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, pig, rabbit, bovine |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | v-akt murine thymoma viral oncogene homolog 1 |
Synonyms | AKT (Ser473), p AKT, p AKT (Ser473), p-AKT, p-AKT (Ser473) |
Observed Molecular Weight | 58 kDa |
GenBank Accession Number | NM_005163 |
Gene Symbol | AKT1 |
Gene ID (NCBI) | 207 |
RRID | AB_2918892 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P31749 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-AKT (Ser473) antibody 80455-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
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