验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, A375 cells, human brain tissue, HeLa cells, MCF-7 cells, RAW 264.7 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human liver tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-87 MG cells |
Planning an IHC experiment? We recommend our IHCeasy RAB35 Ready-To-Use IHC Kit. RAB35 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11329-2-AP targets RAB35 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, canine |
| 免疫原 | RAB35 fusion protein Ag1872 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB35, member RAS oncogene family |
| 别名 | Ras-related protein Rab-1C, Ras related protein Rab 35, Ras related protein Rab 1C, RAB1C, GTP-binding protein RAY |
| 计算分子量 | 201 aa, 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC015931 |
| 基因名称 | RAB35 |
| Gene ID (NCBI) | 11021 |
| RRID | AB_2238179 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15286 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB35 is a member of the small GTPase superfamily. Like the other Rab proteins, RAB35 has 2 consecutive cysteine residues at its C terminus, and this region is thought to be involved in membrane association. Rab35 is found on the cell surface and endosomes, where it has been proposed to play a role in recycling of the transferrin and other receptors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB35 antibody 11329-2-AP | Download protocol |
| IHC protocol for RAB35 antibody 11329-2-AP | Download protocol |
| IF protocol for RAB35 antibody 11329-2-AP | Download protocol |
| IP protocol for RAB35 antibody 11329-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain. | ||
Nat Cell Biol Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1. | ||
Nat Commun A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.
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