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  • KD/KO Validated

RAB35 Polyclonal antibody

RAB35 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 11329-2-AP

Print datasheet

Synonyms

Ras-related protein Rab-1C, Ras related protein Rab 35, Ras related protein Rab 1C, RAB1C, GTP-binding protein RAY



经过测试的应用

Positive WB detected inA431 cells, A375 cells, human brain tissue, HeLa cells, MCF-7 cells, RAW 264.7 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-87 MG cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11329-2-AP targets RAB35 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine
Immunogen RAB35 fusion protein Ag1872 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB35, member RAS oncogene family
Synonyms Ras-related protein Rab-1C, Ras related protein Rab 35, Ras related protein Rab 1C, RAB1C, GTP-binding protein RAY
Calculated Molecular Weight 201 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC015931
Gene Symbol RAB35
Gene ID (NCBI) 11021
RRIDAB_2238179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15286
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB35 is a member of the small GTPase superfamily. Like the other Rab proteins, RAB35 has 2 consecutive cysteine residues at its C terminus, and this region is thought to be involved in membrane association. Rab35 is found on the cell surface and endosomes, where it has been proposed to play a role in recycling of the transferrin and other receptors.

实验方案

Product Specific Protocols
WB protocol for RAB35 antibody 11329-2-APDownload protocol
IHC protocol for RAB35 antibody 11329-2-APDownload protocol
IF protocol for RAB35 antibody 11329-2-APDownload protocol
IP protocol for RAB35 antibody 11329-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Science

Identification of an oncogenic RAB protein.

Authors - Douglas B Wheeler
  • KD Validated
humanWB

Cell Metab

Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.

Authors - Chaitali Mukherjee
humanWB

Nat Cell Biol

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.

Authors - James W Clancy
humanWB

Nat Cell Biol

Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1.

Authors - Alexander Wallroth
mouseWB,IF

Sci Adv

LRRK2 mediates tubulation and vesicle sorting from lysosomes.

Authors - Luis Bonet-Ponce
human,mouseWB,IHC,IF,CoIP

Nat Commun

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Authors - Corallino Salvatore
  • KD Validated