验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, K-562 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung squamous cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
15105-1-AP targets RABEPK/p40 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | RABEPK/p40 fusion protein Ag2593 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rab9 effector protein with kelch motifs |
| 别名 | p40, RABEPK, RAB9P40, Rab9 effector protein with kelch motifs, 40 kDa Rab9 effector protein |
| 计算分子量 | 41 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC065725 |
| 基因名称 | RABEPK/p40 |
| Gene ID (NCBI) | 10244 |
| RRID | AB_1557623 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z6M1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RABEPK/p40 antibody 15105-1-AP | Download protocol |
| IHC protocol for RABEPK/p40 antibody 15105-1-AP | Download protocol |
| IF protocol for RABEPK/p40 antibody 15105-1-AP | Download protocol |
| IP protocol for RABEPK/p40 antibody 15105-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
