验证数据展示
经过测试的应用
| Positive WB detected in | human RPE cells |
| Positive IHC detected in | human brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
15017-1-AP targets IFT27 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | IFT27 fusion protein Ag6967 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB, member of RAS oncogene family-like 4 |
| 别名 | RABL4, Rab-like protein 4, Rab like protein 4, Putative GTP-binding protein RAY-like, BBS19 |
| 计算分子量 | 20 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC000566 |
| 基因名称 | RABL4/IFT27 |
| Gene ID (NCBI) | 11020 |
| RRID | AB_2878100 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BW83 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RABL4, alo named as IFT27, is a GTP-binding protein that is a core component of the intraflagellar transport complex B. It plays a role in the cargo loading of the retrograde transport machinery.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for IFT27 antibody 15017-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.
| ||
Mol Cell Proteomics CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement. | ||
Anal Chem Targeted Proteomic Analysis of Small GTPases in Radioresistant Breast Cancer Cells |
