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  • Featured Product
  • KD/KO Validated

IFT27 Polyclonal antibody

IFT27 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15017-1-AP

Print datasheet

Synonyms

RABL4, Rab-like protein 4, Rab like protein 4, Putative GTP-binding protein RAY-like, BBS19

验证数据展示

  • WB analysis of human RPE cells using 15017-1-AP

    WB analysis of human RPE cells using 15017-1-AP

    WB result of anti-RABL4 (15017-1-AP) with human RPE cells (siRNA and control) by Dr. Gerald Liew, Maxence Nachury's lab.

  • IF Staining of hTERT-RPE1 cells using 15017-1-AP

    IF Staining of hTERT-RPE1 cells using 15017-1-AP

    IF result (cytoplasm and the spindle midzone stain) of anti-RABL4 (IFT27, 15017-1-AP, 1:50) with telophase hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.

  • IHC staining of human brain using 15017-1-AP

    IHC staining of human brain using 15017-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 15017-1-AP

    IHC staining of human brain using 15017-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 15017-1-AP

    IHC staining of human testis using 15017-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 15017-1-AP

    IHC staining of human testis using 15017-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman RPE cells
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15017-1-AP targets IFT27 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen IFT27 fusion protein Ag6967 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB, member of RAS oncogene family-like 4
Synonyms RABL4, Rab-like protein 4, Rab like protein 4, Putative GTP-binding protein RAY-like, BBS19
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC000566
Gene Symbol RABL4/IFT27
Gene ID (NCBI) 11020
RRIDAB_2878100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BW83
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RABL4, alo named as IFT27, is a GTP-binding protein that is a core component of the intraflagellar transport complex B. It plays a role in the cargo loading of the retrograde transport machinery.

实验方案

Product Specific Protocols
IHC protocol for IFT27 antibody 15017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.

Authors - Malavika Raman
  • KD Validated
View Article
humanWB

Mol Cell Proteomics

CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement.

Authors - Tina Beyer
View Article
humanWB

Anal Chem

Targeted Proteomic Analysis of Small GTPases in Radioresistant Breast Cancer Cells

Authors - Zi Gao
View Article