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  • Featured Product
  • KD/KO Validated

IFT27 Polyclonal antibody

IFT27 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 15017-1-AP

Print datasheet

Synonyms

IFT27, Rab like protein 4, RABL4, RAYL

验证数据展示

  • WB analysis of human RPE cells using 15017-1-AP

    WB analysis of human RPE cells using 15017-1-AP

    WB result of anti-RABL4 (15017-1-AP) with human RPE cells (siRNA and control) by Dr. Gerald Liew, Maxence Nachury's lab.

  • IF Staining of hTERT-RPE1 cells using 15017-1-AP

    IF Staining of hTERT-RPE1 cells using 15017-1-AP

    IF result (cytoplasm and the spindle midzone stain) of anti-RABL4 (IFT27, 15017-1-AP, 1:50) with telophase hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.

  • IHC staining of human brain using 15017-1-AP

    IHC staining of human brain using 15017-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 15017-1-AP

    IHC staining of human brain using 15017-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 15017-1-AP

    IHC staining of human testis using 15017-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 15017-1-AP

    IHC staining of human testis using 15017-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman RPE cells
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15017-1-AP targets IFT27 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen IFT27 fusion protein Ag6967 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB, member of RAS oncogene family-like 4
Synonyms IFT27, Rab like protein 4, RABL4, RAYL
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC000566
Gene Symbol RABL4
Gene ID (NCBI) 11020
RRIDAB_2878100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BW83
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RABL4, alo named as IFT27, is a GTP-binding protein that is a core component of the intraflagellar transport complex B. It plays a role in the cargo loading of the retrograde transport machinery.

实验方案

Product Specific Protocols
IHC protocol for IFT27 antibody 15017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.

Authors - Malavika Raman
  • KD Validated
View Article
humanWB

Mol Cell Proteomics

CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement.

Authors - Tina Beyer
View Article
humanWB

Anal Chem

Targeted Proteomic Analysis of Small GTPases in Radioresistant Breast Cancer Cells

Authors - Zi Gao
View Article