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Rac1 Polyclonal antibody
Rac1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (2)
IF, IHC, WB, ELISA and More (2)
Cat No : 24072-1-AP
|Positive WB detected in||mouse brain tissue, human brain tissue, rat brain tissue|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
24072-1-AP targets Rac1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Applications||IF, IHC, WB, ELISA|
|Cited Applications||coIP, IF, IHC, IP, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken, hamster|
|Immunogen||Rac1 fusion protein Ag17779 种属同源性预测|
|Host / Isotype||Rabbit / IgG|
|Full Name||ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)|
|Synonyms||MIG5, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC004247|
|Gene ID (NCBI)||5879|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
|Product Specific Protocols|
|WB protocol for Rac1 antibody 24072-1-AP||Download protocol|
|IHC protocol for Rac1 antibody 24072-1-AP||Download protocol|
|IF protocol for Rac1 antibody 24072-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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