RAD1 Polyclonal antibody

RAD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11726-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA

REC1, Rad1-like DNA damage checkpoint protein, HRAD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, mouse spleen tissue, K-562 cells, L02 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

11726-2-AP targets RAD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, rat, rainbow trout
免疫原 RAD1 fusion protein Ag2316 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAD1 homolog (S. pombe)
别名 REC1, Rad1-like DNA damage checkpoint protein, HRAD1
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC006837
基因名称 RAD1
Gene ID (NCBI) 5810
RRIDAB_2246457
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60671
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD1, cell cycle checkpoint protein, is a component of a heterotrimeric cell cycle checkpoint 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. RAD1, HUS1 and RAD9 are central components of a DNA damage-responsive protein complex. Testis-specific disruption of RAD1 in mice resulted in defective double-strand break repair, depletion of germ cells and infertility (PMID: 35133274). RAD1 has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for RAD1 antibody 11726-2-APDownload protocol
IHC protocol for RAD1 antibody 11726-2-APDownload protocol
IF protocol for RAD1 antibody 11726-2-APDownload protocol
IP protocol for RAD1 antibody 11726-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncogene

DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.

Authors - Bin Chen
humanWB

J Mol Cell Biol

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Authors - Xiaoli Xu
humanWB

Am J Physiol Cell Physiol

Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability.

Authors - Rongyi Shi
rainbow troutWB

Comp Biochem Physiol C Toxicol Pharmacol

The rad1 gene in Rainbow Trout (Oncorhynchus mykiss) is highly conserved and may express proteins from non-canonical spliced isoforms.

Authors - Bozdarov Johny J
ratWB

Mol Med

Rad1 attenuates DNA double-strand breaks and cell cycle arrest in type II alveolar epithelial cells of rats with bronchopulmonary dysplasia

Authors - Xin Tong
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