验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A549 cells, MCF-7 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 5 publications below |
IHC | See 1 publications below |
产品信息
13035-1-AP targets RAD9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | RAD9A fusion protein Ag3687 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | RAD9 homolog A (S. pombe) |
别名 | RAD9, hRAD9, EC:3.1.11.2, Cell cycle checkpoint control protein RAD9A |
计算分子量 | 391 aa, 42 kDa |
观测分子量 | 55-60 kDa |
GenBank蛋白编号 | BC014848 |
基因名称 | RAD9A |
Gene ID (NCBI) | 5883 |
RRID | AB_10598474 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q99638 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for RAD9A antibody 13035-1-AP | Download protocol |
IHC protocol for RAD9A antibody 13035-1-AP | Download protocol |
IF protocol for RAD9A antibody 13035-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
EMBO J YTHDC1 delays cellular senescence and pulmonary fibrosis by activating ATR in an m6A-independent manner | ||
Oncogene DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity. | ||
J Mol Cell Biol SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress. | ||
DNA Cell Biol AEG-1 Knockdown Sensitizes Glioma Cells to Radiation Through Impairing Homologous Recombination Via Targeting RFC5. | ||
Int Immunopharmacol Unveiling an anoikis-related risk model and the role of RAD9A in colon cancer
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