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- KD/KO Validated
RBM15B Polyclonal antibody
RBM15B Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IP, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, mouse brain tissue |
| Positive IP detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy RBM15B Ready-To-Use IHC Kit. RBM15B primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 22249-1-AP is RBM15B Fusion Protein expressed in E. coli.
| Tested Applications | IP, WB, ELISA |
| Cited Applications | CoIP, IHC, RIP, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | RBM15B fusion protein Ag17744 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | RNA binding motif protein 15B |
| Synonyms | HUMAGCGB, HuOTT3, One twenty two protein 3, OTT3, RBM15B, RNA binding motif protein 15B |
| Calculated Molecular Weight | 890 aa, 97 kDa |
| Observed Molecular Weight | 100-120 kDa, 70 kDa |
| GenBank Accession Number | BC001367 |
| Gene Symbol | RBM15B |
| Gene ID (NCBI) | 29890 |
| RRID | AB_2879048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NDT2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa), thus this antibody can recognize full length of RBM15B(~100kDa) and splice-one(70kDa)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBM15B antibody 22249-1-AP | Download protocol |
| IP protocol for RBM15B antibody 22249-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis. | ||
Elife SNORD90 induces glutamatergic signaling following treatment with monoaminergic antidepressants | ||
Front Genet m6A Regulatory Gene-Mediated Methylation Modification in Glioma Survival Prediction. | ||
Med Sci Monit Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma. |
