验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14895-1-AP targets RBX1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, eriocheir sinensis |
| 免疫原 |
CatNo: Ag6685 Product name: Recombinant human RBX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-108 aa of BC001466 Sequence: MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring-box 1 |
| 别名 | BA554C12.1, E3 ubiquitin-protein ligase RBX1, E3 ubiquitin-protein ligase RBX1, N-terminally processed, E3 ubiquitin-protein transferase RBX1, EC:2.3.2.27 |
| 计算分子量 | 12 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC001466 |
| 基因名称 | RBX1 |
| Gene ID (NCBI) | 9978 |
| RRID | AB_2179719 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62877 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40.It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RBX1 antibody 14895-1-AP | Download protocol |
| IP protocol for RBX1 antibody 14895-1-AP | Download protocol |
| WB protocol for RBX1 antibody 14895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab UBE2M-mediated neddylation of TRIM21 regulates obesity-induced inflammation and metabolic disorders | ||
Cancer Commun (Lond) HMMR alleviates endoplasmic reticulum stress by promoting autophagolysosomal activity during endoplasmic reticulum stress-driven hepatocellular carcinoma progression | ||
Nat Cell Biol Phase separation of ERCC6L2-CtIP regulates the extent of DNA end resection
| ||
Adv Sci (Weinh) The Crosstalk Between CRL5 and APC/C E3 Ligases Regulates Metastasis and Chemosensitivity of Cancer Cells. | ||
Cell Death Differ USP51 facilitates colorectal cancer stemness and chemoresistance by forming a positive feed-forward loop with HIF1A | ||
Cell Death Differ CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation. |
