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NELFE Polyclonal antibody

NELFE Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10705-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

RNA-binding protein RD, RDBP, RD, NELF-E, NELF E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue, mouse eye tissue, mouse placenta tissue, mouse kidney tissue, rat kidney tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inhela cells
Planning an IHC experiment? We recommend our IHCeasy NELFE Ready-To-Use IHC Kit. NELFE primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10705-1-AP targets NELFE in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, ChIP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 NELFE fusion protein Ag1186 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RD RNA binding protein
别名 RNA-binding protein RD, RDBP, RD, NELF-E, NELF E
计算分子量 37 kDa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC011600
基因名称 NELFE
Gene ID (NCBI) 7936
RRIDAB_513966
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18615
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

实验方案

Product Specific Protocols
WB protocol for NELFE antibody 10705-1-APDownload protocol
IHC protocol for NELFE antibody 10705-1-APDownload protocol
IF protocol for NELFE antibody 10705-1-APDownload protocol
IP protocol for NELFE antibody 10705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nature

The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.

Authors - Bofeng Liu
human, mouseWB

Cell

Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.

Authors - Sawarkar Ritwick R
mouseWB,chIP

Nat Commun

Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.

Authors - Li Yu
mouseWB

Nat Commun

RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity.

Authors - Bogang Wu
mouseWB

Dev Cell

Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.

Authors - Daniel C L Robinson
mouseWB,IHC

Proc Natl Acad Sci U S A

Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.

Authors - Adelman Karen K
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