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  • Featured Product
  • KD/KO Validated

REDD1 specific Polyclonal antibody

REDD1 specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 10638-1-AP

Print datasheet

Synonyms

DDIT4, REDD1, RTP801, REDD-1, HIF-1 responsive protein RTP801

验证数据展示

  • WB analysis using 10638-1-AP

    WB analysis using 10638-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 cells using 10638-1-AP

    WB analysis of PC-3 cells using 10638-1-AP

    WB result of REDD1 antibody (10638-1-AP, 1:1000) with si-control and si-REDD1 transfected PC-3 cells.

  • WB analysis using 10638-1-AP

    WB analysis using 10638-1-AP

    Non-treated Hela and Cobalt Chloride treated Hela cells were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 incubated at room temperature for 6 hours. .

  • WB analysis using 10638-1-AP

    WB analysis using 10638-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 10638-1-AP

    IP experiment of MCF-7 using 10638-1-AP

    IP result of anti-REDD1 specific (IP:10638-1-AP, 3ug; Detection:10638-1-AP 1:500) with MCF-7 cells lysate 2500ug.

  • IHC staining of human lung cancer using 10638-1-AP

    IHC staining of human lung cancer using 10638-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 10638-1-AP

    IHC staining of mouse brain using 10638-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 10638-1-AP

    IHC staining of mouse brain using 10638-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 10638-1-AP

    WB analysis using 10638-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of LNCaP using 10638-1-AP

    IF Staining of LNCaP using 10638-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using REDD1 specific antibody (10638-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, DU 145 cells, Cobalt Chloride treated HeLa cells, LNCaP cells, PC-3 cells, A549 cells, K-562 cells, SH-SY5Y cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inLNCaP cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10638-1-AP targets REDD1 specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, chIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, rabbit, squirrel, gerbil
Immunogen REDD1 specific fusion protein Ag0965 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DNA-damage-inducible transcript 4
Synonyms DDIT4, REDD1, RTP801, REDD-1, HIF-1 responsive protein RTP801
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberBC007714
Gene Symbol REDD1/DDIT4
Gene ID (NCBI) 54541
RRIDAB_2245711
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX09
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific to the REDD1 from siRNA experiment (PMID:24713927)

实验方案

Product Specific Protocols
WB protocol for REDD1 specific antibody 10638-1-APDownload protocol
IHC protocol for REDD1 specific antibody 10638-1-APDownload protocol
IF protocol for REDD1 specific antibody 10638-1-APDownload protocol
IP protocol for REDD1 specific antibody 10638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

Plzf regulates germline progenitor self-renewal by opposing mTORC1.

Authors - Hobbs Robin M RM
View Article
humanWB

Cell

Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR.

Authors - Zandra E Walton
View Article
mouseWB

Cancer Cell

A UBE2O-AMPKα2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.

Authors - Isabelle K Vila
View Article
ratWB

Nat Med

REDD1 is essential for stress-induced synaptic loss and depressive behavior.

Authors - Kristie T Ota
View Article
mousechIP

Nat Immunol

The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity.

Authors - Wilson Castro
View Article
mouseWB

Acta Neuropathol

MEF2 impairment underlies skeletal muscle atrophy in polyglutamine disease.

Authors - Samir R Nath
View Article