验证数据展示

WB analysis using 10638-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 cells using 10638-1-AP
WB result of REDD1 antibody (10638-1-AP, 1:1000) with si-control and si-REDD1 transfected PC-3 cells.
WB analysis using 10638-1-AP
Non-treated Hela and Cobalt Chloride treated Hela cells were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 incubated at room temperature for 6 hours. .
WB analysis using 10638-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 10638-1-AP
IP result of anti-REDD1 specific (IP:10638-1-AP, 3ug; Detection:10638-1-AP 1:500) with MCF-7 cells lysate 2500ug.
IHC staining of human lung cancer using 10638-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10638-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10638-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB analysis using 10638-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IF Staining of LNCaP using 10638-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using REDD1 specific antibody (10638-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

REDD1 specific Polyclonal antibody

REDD1 specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10638-1-AP

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Publications(275)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Synonyms

DDIT4, REDD1, RTP801, REDD-1, HIF-1 responsive protein RTP801

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (275)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	A431 cells, DU 145 cells, Cobalt Chloride treated HeLa cells, LNCaP cells, PC-3 cells, A549 cells, K-562 cells, SH-SY5Y cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human lung cancer tissue, human heart tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	LNCaP cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 38 publications below
WB	See 256 publications below
IHC	See 25 publications below
IF	See 18 publications below
IP	See 1 publications below
FC	See 1 publications below
CoIP	See 3 publications below
ChIP	See 2 publications below

产品信息

10638-1-AP targets REDD1 specific in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP, chIP
Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, rabbit, squirrel, gerbil
Immunogen	REDD1 specific fusion protein Ag0965 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	DNA-damage-inducible transcript 4
Synonyms	DDIT4, REDD1, RTP801, REDD-1, HIF-1 responsive protein RTP801
Calculated Molecular Weight	25 kDa
Observed Molecular Weight	32-35 kDa
GenBank Accession Number	BC007714
Gene Symbol	REDD1/DDIT4
Gene ID (NCBI)	54541
RRID	AB_2245711
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NX09
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific to the REDD1 from siRNA experiment (PMID:24713927)

实验方案

Product Specific Protocols
WB protocol for REDD1 specific antibody 10638-1-AP	Download protocol
IHC protocol for REDD1 specific antibody 10638-1-AP	Download protocol
IF protocol for REDD1 specific antibody 10638-1-AP	Download protocol
IP protocol for REDD1 specific antibody 10638-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Cell Plzf regulates germline progenitor self-renewal by opposing mTORC1. Authors - Hobbs Robin M RM View Article
human	WB	Cell Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR. Authors - Zandra E Walton View Article
human,mouse	WB	Cell Metab Disrupted methionine cycle triggers muscle atrophy in cancer cachexia through epigenetic regulation of REDD1 Authors - Kai Lin View Article
mouse	WB	Cancer Cell A UBE2O-AMPKα2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy. Authors - Isabelle K Vila View Article
rat	WB	Nat Med REDD1 is essential for stress-induced synaptic loss and depressive behavior. Authors - Kristie T Ota View Article
mouse	chIP	Nat Immunol The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity. Authors - Wilson Castro View Article

验证数据展示

WB analysis using 10638-1-AP

WB analysis of PC-3 cells using 10638-1-AP

WB analysis using 10638-1-AP

WB analysis using 10638-1-AP

IP experiment of MCF-7 using 10638-1-AP

IHC staining of human lung cancer using 10638-1-AP

IHC staining of mouse brain using 10638-1-AP

IHC staining of mouse brain using 10638-1-AP

WB analysis using 10638-1-AP

IF Staining of LNCaP using 10638-1-AP

REDD1 specific Polyclonal antibody

REDD1 specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

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经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Plzf regulates germline progenitor self-renewal by opposing mTORC1.

Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR.

Disrupted methionine cycle triggers muscle atrophy in cancer cachexia through epigenetic regulation of REDD1

A UBE2O-AMPKα2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.

REDD1 is essential for stress-induced synaptic loss and depressive behavior.

The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity.