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REDD1 specific Polyclonal antibody

REDD1 specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10638-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (5)

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

DDIT4, REDD1, RTP801, REDD-1, HIF-1 responsive protein RTP801

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, DU 145 cells, Cobalt Chloride treated HeLa cells, LNCaP cells, PC-3 cells, A549 cells, K-562 cells, SH-SY5Y cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inLNCaP cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10638-1-AP targets REDD1 specific in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, chIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, pig, rabbit, squirrel, gerbil
免疫原 REDD1 specific fusion protein Ag0965 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DNA-damage-inducible transcript 4
别名 DDIT4, REDD1, RTP801, REDD-1, HIF-1 responsive protein RTP801
计算分子量 25 kDa
观测分子量 32-35 kDa
GenBank蛋白编号BC007714
基因名称 REDD1/DDIT4
Gene ID (NCBI) 54541
RRIDAB_2245711
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NX09
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific to the REDD1 from siRNA experiment (PMID:24713927)

实验方案

Product Specific Protocols
WB protocol for REDD1 specific antibody 10638-1-APDownload protocol
IHC protocol for REDD1 specific antibody 10638-1-APDownload protocol
IF protocol for REDD1 specific antibody 10638-1-APDownload protocol
IP protocol for REDD1 specific antibody 10638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

Plzf regulates germline progenitor self-renewal by opposing mTORC1.

Authors - Hobbs Robin M RM
humanWB

Cell

Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR.

Authors - Zandra E Walton
human,mouseWB

Cell Metab

Disrupted methionine cycle triggers muscle atrophy in cancer cachexia through epigenetic regulation of REDD1

Authors - Kai Lin
mouseWB

Cancer Cell

A UBE2O-AMPKα2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.

Authors - Isabelle K Vila
ratWB

Nat Med

REDD1 is essential for stress-induced synaptic loss and depressive behavior.

Authors - Kristie T Ota
mousechIP

Nat Immunol

The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity.

Authors - Wilson Castro
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