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  • Featured Product
  • KD/KO Validated

REDD1 specific Polyclonal antibody

REDD1 specific Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

IHC, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 10638-1-AP

Print datasheet

Synonyms

DDIT4, Dig2, REDD 1, REDD1, REDD1 specific, RP11 442H21.1, RTP801

验证数据展示

  • WB analysis using 10638-1-AP

    WB analysis using 10638-1-AP

    Non-treated Hela and Cobalt Chloride treated Hela cells were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 incubated at room temperature for 6 hours. .

  • WB analysis of PC-3 cells using 10638-1-AP

    WB analysis of PC-3 cells using 10638-1-AP

    WB result of REDD1 antibody (10638-1-AP, 1:1000) with si-control and si-REDD1 transfected PC-3 cells.

  • WB analysis using 10638-1-AP

    WB analysis using 10638-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10638-1-AP

    WB analysis using 10638-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 10638-1-AP

    IP experiment of MCF-7 using 10638-1-AP

    IP result of anti-REDD1 specific (IP:10638-1-AP, 3ug; Detection:10638-1-AP 1:500) with MCF-7 cells lysate 2500ug.

  • WB analysis using 10638-1-AP

    WB analysis using 10638-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 10638-1-AP

    IHC staining of human lung cancer using 10638-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 10638-1-AP

    IHC staining of mouse brain using 10638-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 10638-1-AP

    IHC staining of mouse brain using 10638-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of LNCaP using 10638-1-AP

    IF Staining of LNCaP using 10638-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using REDD1 specific antibody (10638-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, DU 145 cells, Cobalt Chloride treated HeLa cells, LNCaP cells, PC-3 cells, A549 cells, K-562 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10638-1-AP targets REDD1 specific in WB, IP, IF, FC, IHC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IP, IF, FC, IHC, CoIP, chIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, rabbit, pig, squirrel, gerbil
Immunogen REDD1 specific fusion protein Ag0965 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DNA-damage-inducible transcript 4
Synonyms DDIT4, Dig2, REDD 1, REDD1, REDD1 specific, RP11 442H21.1, RTP801
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberBC007714
Gene Symbol DDIT4
Gene ID (NCBI) 54541
RRIDAB_2245711
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX09
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific to the REDD1 from siRNA experiment (PMID:24713927)

实验方案

Product Specific Protocols
WB protocol for REDD1 specific antibody 10638-1-APDownload protocol
IHC protocol for REDD1 specific antibody 10638-1-APDownload protocol
IP protocol for REDD1 specific antibody 10638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

Plzf regulates germline progenitor self-renewal by opposing mTORC1.

Authors - Hobbs Robin M RM
View Article
humanWB

Cell

Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR.

Authors - Zandra E Walton
View Article
mouseWB

Cancer Cell

A UBE2O-AMPKα2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy.

Authors - Isabelle K Vila
View Article
ratWB

Nat Med

REDD1 is essential for stress-induced synaptic loss and depressive behavior.

Authors - Kristie T Ota
View Article
mousechIP

Nat Immunol

The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity.

Authors - Wilson Castro
View Article
mouseWB

Acta Neuropathol

MEF2 impairment underlies skeletal muscle atrophy in polyglutamine disease.

Authors - Samir R Nath
View Article