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REG Polyclonal antibody

REG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15850-1-AP

Print datasheet

Synonyms

REG1 alpha, REG1A, Pancreatic thread protein, Pancreatic stone protein, P19

验证数据展示

  • WB analysis using 15850-1-AP

    WB analysis using 15850-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15850-1-AP (REG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 15850-1-AP

    IHC staining of human stomach cancer using 15850-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 15850-1-AP (REG antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 15850-1-AP

    IHC staining of human stomach cancer using 15850-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15850-1-AP (REG antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse pancreas tissue, rat pancreas tissue
Positive IHC detected inhuman stomach cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15850-1-AP targets REG in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen REG fusion protein Ag8640 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regenerating islet-derived 1 alpha
Synonyms REG1 alpha, REG1A, Pancreatic thread protein, Pancreatic stone protein, P19
Calculated Molecular Weight 166 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC005350
Gene Symbol REG
Gene ID (NCBI) 5967
RRIDAB_2878192
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05451
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for REG antibody 15850-1-APDownload protocol
IHC protocol for REG antibody 15850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int Immunopharmacol

Protective and anti-inflammatory role of REG1A in inflammatory bowel disease induced by JAK/STAT3 signaling axis.

Authors - Hongli Mao
View Article
human,mouseWB,IF

Ann N Y Acad Sci

REG1A protects retinal photoreceptors from blue light damage

Authors - Ze-Hua Xu
View Article