验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, NIH/3T3 cells, HeLa cells, HepG2 cells |
Positive IHC detected in | human lung cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | NIH/3T3 cells, HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27248-1-AP targets RICTOR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse, rat |
免疫原 | RICTOR fusion protein Ag25649 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | rapamycin-insensitive companion of mTOR |
别名 | Rapamycin-insensitive companion of mTOR, KIAA1999, hAVO3, AVO3 homolog |
计算分子量 | 192 kDa |
观测分子量 | 192 kDa |
GenBank蛋白编号 | BC029608 |
基因名称 | RICTOR |
Gene ID (NCBI) | 253260 |
RRID | AB_2880817 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q6R327 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for RICTOR antibody 27248-1-AP | Download protocol |
IHC protocol for RICTOR antibody 27248-1-AP | Download protocol |
IF protocol for RICTOR antibody 27248-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Aging (Albany NY) Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts | ||
Cell Rep Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways | ||
J Cell Physiol Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes | ||
J Ethnopharmacol Diterpenoid tanshinones inhibit gastric cancer angiogenesis through the PI3K/Akt/mTOR signaling pathway | ||
Cell Commun Signal Rapamycin promotes endothelial-mesenchymal transition during stress-induced premature senescence through the activation of autophagy. |