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RICTOR Polyclonal antibody

RICTOR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27248-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (2)

Rapamycin-insensitive companion of mTOR, KIAA1999, hAVO3, AVO3 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RICTOR Ready-To-Use IHC Kit. RICTOR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27248-1-AP targets RICTOR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 RICTOR fusion protein Ag25649 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 rapamycin-insensitive companion of mTOR
别名 Rapamycin-insensitive companion of mTOR, KIAA1999, hAVO3, AVO3 homolog
计算分子量 192 kDa
观测分子量 192 kDa
GenBank蛋白编号BC029608
基因名称 RICTOR
Gene ID (NCBI) 253260
RRIDAB_2880817
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6R327
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RICTOR antibody 27248-1-APDownload protocol
IHC protocol for RICTOR antibody 27248-1-APDownload protocol
IF protocol for RICTOR antibody 27248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Neurosci

A glycolytic shift in Schwann cells supports injured axons.

Authors - Elisabetta Babetto
humanWB

Aging (Albany NY)

Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts

Authors - Shi-Yu Wang
mouseWB,IF

Cell Rep

Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways

Authors - Tatsuki Yasuda
mouseIF

J Cell Physiol

Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes

Authors - Rui Hua
humanWB

J Ethnopharmacol

Diterpenoid tanshinones inhibit gastric cancer angiogenesis through the PI3K/Akt/mTOR signaling pathway

Authors - Jie-Ru Yu
humanWB

Cell Commun Signal

Rapamycin promotes endothelial-mesenchymal transition during stress-induced premature senescence through the activation of autophagy.

Authors - Norihiko Sasaki
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