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  • Featured Product
  • KD/KO Validated

ROCK1-Specific Polyclonal antibody

ROCK1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20247-1-AP

Print datasheet

Synonyms

p160 ROCK 1, P160ROCK, PRO0435, ROCK1, ROCK1-Specific

验证数据展示

  • WB analysis of HeLa using 20247-1-AP

    WB analysis of HeLa using 20247-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20247-1-AP (ROCK1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 20247-1-AP

    IHC staining of human lung cancer using 20247-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20247-1-AP (ROCK1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20247-1-AP targets ROCK1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho-associated, coiled-coil containing protein kinase 1
Synonyms p160 ROCK 1, P160ROCK, PRO0435, ROCK1, ROCK1-Specific
Calculated Molecular Weight 158 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberNM_005406
Gene Symbol ROCK1
Gene ID (NCBI) 6093
RRIDAB_10694275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13464
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to ROCK1.

实验方案

Product Specific Protocols
WB protocol for ROCK1-Specific antibody 20247-1-APDownload protocol
IHC protocol for ROCK1-Specific antibody 20247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

ASN Neuro

Induction of oligodendrocyte differentiation and in vitro myelination by inhibition of rho-associated kinase.

Authors - Carlos E Pedraza
  • KD Validated
View Article