验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, BxPC-3 cells, mouse lung tissue, mouse pancreas tissue, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
Planning an IHC experiment? We recommend our IHCeasy RPL11 Ready-To-Use IHC Kit. RPL11 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16277-1-AP targets RPL11 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RPL11 fusion protein Ag9356 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L11 |
| 别名 | 60S ribosomal protein L11, CLL associated antigen KW 12, DBA7, GIG34, ribosomal protein L11, RPL11 |
| 计算分子量 | 177 aa, 20 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC018970 |
| 基因名称 | RPL11 |
| Gene ID (NCBI) | 6135 |
| RRID | AB_2181292 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62913 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomal protein L11 is one of 80 mammalian ribosome proteins. It can bind to 5S ribosomal RNA, for it's required for rRNA maturation and formation of the 60S ribosomal subunit. It can inhibit 40S or 60S ribosome biogenesis via mediated p53 induction. Once retained by PICT1 in the nucleolus, RPL11 involve in regulation of the MDM2-P53 pathway and promotion of tumor progression.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL11 antibody 16277-1-AP | Download protocol |
| IHC protocol for RPL11 antibody 16277-1-AP | Download protocol |
| IF protocol for RPL11 antibody 16277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling. | ||
Nat Commun Cilia locally synthesize proteins to sustain their ultrastructure and functions. | ||
Am J Hum Genet Functional analysis of the 1p34.3 risk locus implicates GNL2 in high-grade serous ovarian cancer. | ||
Dev Cell Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility. | ||
Curr Biol RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. |
