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RPL5 Polyclonal antibody

RPL5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Monkey and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29092-1-AP

Print datasheet

Synonyms

60S ribosomal protein L5, DBA6, MSTP030, ribosomal protein L5, RPL5

验证数据展示

  • WB analysis using 29092-1-AP

    WB analysis using 29092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29092-1-AP (RPL5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 29092-1-AP

    IHC staining of human prostate cancer using 29092-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29092-1-AP (RPL5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCOS-7 cells, MOLT-4 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29092-1-AP targets RPL5 in WB, IHC, ELISA applications and shows reactivity with Human, Monkey samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human, Monkey
Cited Reactivityhuman
Immunogen RPL5 fusion protein Ag30837 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L5
Synonyms 60S ribosomal protein L5, DBA6, MSTP030, ribosomal protein L5, RPL5
Observed Molecular Weight34 kDa
GenBank Accession NumberBC109370
Gene Symbol RPL5
Gene ID (NCBI) 6125
RRIDAB_2881240
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46777
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian ribosome is a macromolecular assembly of 4 RNA species (rRNAs) and approximately 80 different proteins, including RPL5. RPL5 (ribosome protein L15) is rquired for rRNA maturation and formation of the 60S ribosomal subunits, and defects or mutation in RPL5 is the cause of DBA6.

实验方案

Product Specific Protocols
WB protocol for RPL5 antibody 29092-1-APDownload protocol
IHC protocol for RPL5 antibody 29092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

mTOR- and LARP1-dependent regulation of TOP mRNA poly(A) tail and ribosome loading

Authors - Koichi Ogami
View Article
humanWB

BMC Mol Cell Biol

Ribosomal protein RPL5 regulates colon cancer cell proliferation and migration through MAPK/ERK signaling pathway

Authors - Huahua Zhang
  • KD Validated
View Article
humanIHC

Front Oncol

Spatially resolved transcriptomics revealed local invasion-related genes in colorectal cancer

Authors - Hong-Tao Liu
View Article
humanWB

iScience

Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges

Authors - Tamami Miyagi
View Article