RPMI 1640 培养基
Cat No : PM00032
Validation Data Gallery
产品介绍
RPMI 1640培养基最初是为长期培养外周血淋巴细胞而开发的，后来被发现也适用于多种哺乳动物细胞包括：HeLa、Jurkat、MCF-7 、PC12、PBMC、星形胶质细胞和癌细胞等细胞系，尤其适用于悬浮细胞的培养，是使用最为广泛的培养基之一。
RPMI 1640培养基与其它培养基的区别在于含有还原型谷胱甘肽和高浓度的维生素。RPMI 1640培养基含有EMEM和DMEM中没有的生物素、维生素B12和对氨基苯甲酸，以及高浓度的氯化胆碱和肌醇。
本产品含有： D-葡萄糖、酚红、 L-谷氨酰胺。
不含有：丙酮酸钠、 HEPES。
产品内容
| 产品组成 | PM00032 |
RPMI 1640培养基 | 500 ml |
| 说明书 | 1份 |
保存条件
2-8℃避光保存， 一年有效。
产品组分
| Components | Molecular Weight | Concentration (mg/L） | mM |
| Amino Acids | |||
| Glycine | 75.0 | 10.0 | 0.13333334 |
| L-Arginine | 174.0 | 200.0 | 1.1494253 |
| L-Asparagine | 132.0 | 50.0 | 0.37878788 |
L-Aspartic acid | 133.0 | 20.0 | 0.15037593 |
| L-Cystine 2HCl | 313.0 | 65.0 | 0.20766774 |
L-Glutamic Acid | 147.0 | 20.0 | 0.13605443 |
L-Glutamine | 146.0 | 300.0 | 2.0547945 |
| L-Histidine | 155.0 | 15.0 | 0.09677419 |
L-Hydroxyproline | 131.0 | 20.0 | 0.15267175 |
L-Isoleucine | 131.0 | 50.0 | 0.3816794 |
| L-Leucine | 131.0 | 50.0 | 0.3816794 |
L-Lysine hydrochloride | 183.0 | 40.0 | 0.21857923 |
L-Methionine | 149.0 | 15.0 | 0.10067114 |
L-Phenylalanine | 165.0 | 15.0 | 0.09090909 |
L-Proline | 115.0 | 20.0 | 0.17391305 |
| L-Serine | 105.0 | 30.0 | 0.2857143 |
L-Threonine | 119.0 | 20.0 | 0.16806723 |
L-Tryptophan | 204.0 | 5.0 | 0.024509804 |
L-Tyrosine disodium salt dihydrate | 261.0 | 29.0 | 0.11111111 |
L-Valine | 117.0 | 20.0 | 0.17094018 |
| Vitamins | |||
Biotin | 244.0 | 0.2 | 8.1967213E-4 |
| Choline chloride | 140.0 | 3.0 | 0.021428572 |
D-Calcium pantothenate | 477.0 | 0.25 | 5.24109E-4 |
Folic Acid | 441.0 | 1.0 | 0.0022675737 |
| Niacinamide | 122.0 | 1.0 | 0.008196721 |
Para-Aminobenzoic Acid | 137.0 | 1.0 | 0.00729927 |
Pyridoxine hydrochloride | 206.0 | 1.0 | 0.004854369 |
Riboflavin | 376.0 | 0.2 | 5.319149E-4 |
Thiamine hydrochloride | 337.0 | 1.0 | 0.002967359 |
Vitamin B12 | 1355.0 | 0.005 | 3.690037E-6 |
i-Inositol | 180.0 | 35.0 | 0.19444445 |
Inorganic Salt | |||
Calcium nitrate (Ca(NO3)2 4H2O) | 236.0 | 100.0 | 0.42372882 |
Magnesium Sulfate (MgSO4) (anhyd.) | 120.0 | 48.84 | 0.407 |
Potassium Chloride (KCl) | 75.0 | 400.0 | 5.3333335 |
Sodium Chloride (NaCl) | 58.0 | 6000.0 | 103.44827 |
Sodium Phosphate dibasic (Na2HPO4-7H2O) | 268.0 | 800.0 | 2.9850745 |
Sodium Bicarbonate (NaHCO3) | 84.0 | 2000.0 | 23.8 |
| Other Components | |||
D-Glucose (Dextrose) | 180.0 | 2000.0 | 11.111111 |
Glutathione (reduced) | 307.0 | 1.0 | 0.0032573289 |
Phenol Red | 376.4 | 5.0 | 0.013283741 |