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RSK2 Polyclonal antibody

RSK2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

Cat no : 23762-1-AP

Print datasheet

Synonyms

RPS6KA3, ISPK1, ISPK 1, Insulin-stimulated protein kinase 1, HU 3

验证数据展示

WB analysis of K-562 using 23762-1-AP
WB result of RSK2 antibody (23762-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSK2 transfected K-562 cells.
WB analysis using 23762-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 23762-1-AP
IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:4000) with K-562 cells lysate 3680 ug.
IHC staining of human kidney using 23762-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 23762-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 23762-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 23762-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 23762-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 23762-1-AP (RSK2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 23762-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 23762-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 23762-1-AP

Size

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经过测试的应用

Positive WB detected in	NIH/3T3 cells, K-562 cells, MCF-7 cells, HepG2 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human kidney tissue, mouse colon tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue
Positive IF/ICC detected in	HepG2 cells, HUVEC cells

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 7 publications below

产品信息

23762-1-AP targets RSK2 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited Applications	WB
Tested Reactivity	human, mouse
Cited Reactivity	human
Immunogen	RSK2 fusion protein Ag20620 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	ribosomal protein S6 kinase, 90kDa, polypeptide 3
Synonyms	RPS6KA3, ISPK1, ISPK 1, Insulin-stimulated protein kinase 1, HU 3
Calculated Molecular Weight	740 aa, 84 kDa
Observed Molecular Weight	84 kDa
GenBank Accession Number	BC096301
Gene Symbol	RSK2
Gene ID (NCBI)	6197
RRID	AB_2879318
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P51812
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

RPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.

实验方案

Product Specific Protocols
WB protocol for RSK2 antibody 23762-1-AP	Download protocol
IHC protocol for RSK2 antibody 23762-1-AP	Download protocol
IF protocol for RSK2 antibody 23762-1-AP	Download protocol
IP protocol for RSK2 antibody 23762-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
		Theranostics S6K1 phosphorylation-dependent degradation of Mxi1 by β-Trcp ubiquitin ligase promotes Myc activation and radioresistance in lung cancer. Authors - Yumei Huang View Article
human	WB	Cell Death Differ β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer. Authors - Jia Ma View Article
human	WB	Oncogene β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma. Authors - Yanwei Lu View Article
human	WB	Int J Biochem Cell Biol DHPAC, a novel synthetic microtubule destabilizing agent, possess high anti-tumor activity in vincristine-resistant oral epidermoid carcinoma in vitro and in vivo. Authors - Ying Zhang View Article
human	WB	Prostate MicroRNA181c inhibits prostate cancer cell growth and invasion by targeting multiple ERK signaling pathway components. Authors - Zhengzheng Su View Article
human	WB	PLoS One A short peptide LINC00665_18aa encoded by lncRNA LINC00665 suppresses the proliferation and migration of osteosarcoma cells through the regulation of the CREB1/RPS6KA3 interaction Authors - Junwei Pan View Article

RSK2 Polyclonal antibody

RSK2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

验证数据展示

WB analysis of K-562 using 23762-1-AP

WB analysis using 23762-1-AP

IP experiment of K-562 using 23762-1-AP

IHC staining of human kidney using 23762-1-AP

IHC staining of human kidney using 23762-1-AP

IHC staining of mouse colon using 23762-1-AP

IHC staining of mouse kidney using 23762-1-AP

IF Staining of HepG2 using 23762-1-AP

IF Staining of mouse brain using 23762-1-AP

IF Staining of mouse brain using 23762-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

S6K1 phosphorylation-dependent degradation of Mxi1 by β-Trcp ubiquitin ligase promotes Myc activation and radioresistance in lung cancer.

β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer.

β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.

DHPAC, a novel synthetic microtubule destabilizing agent, possess high anti-tumor activity in vincristine-resistant oral epidermoid carcinoma in vitro and in vivo.

MicroRNA181c inhibits prostate cancer cell growth and invasion by targeting multiple ERK signaling pathway components.

A short peptide LINC00665_18aa encoded by lncRNA LINC00665 suppresses the proliferation and migration of osteosarcoma cells through the regulation of the CREB1/RPS6KA3 interaction