Rat IL-2 ELISA Kit
Validation Data Gallery
Product Information
KE20002 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL2. The assay recognizes mouse IL2. An antibody specific for IL2 has been pre-coated onto the microwells. The IL2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL2 is added to detect the captured IL2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat IL-2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 8.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 116562 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 106% | 93%-119% |
| Rat Serum | 97% | 83%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 38.4 | 2.4 | 6.2 |
| 2 | 20 | 219.4 | 9.8 | 4.5 |
| 3 | 20 | 1,013.1 | 62.1 | 6.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 37.0 | 2.3 | 6.2 |
| 2 | 24 | 225.5 | 17.2 | 7.6 |
| 3 | 24 | 996.8 | 84.6 | 8.5 |
Background Information
Interleukin-2 (IL2) is a protein initially identified as a T cell growth factor. IL2 is mainly produced by activated CD4+CD25- (helper) T cells, and induces the proliferation of these and others cells like CD8+ T cells, B and NK cells. For this reason, it has been used to treat immune-deficiencies like HIV, and induce immune response against tumors. Nevertheless, it has been shown that IL2 also acts as the main growth factor of CD4+CD25+ Regulatory T cells. Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (IL2R). IL2R is a multimeric functional protein consisting of three different chains: Il2Rα, Il2Rβ and γc.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il2, Il-2, interleukin 2 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Evid Based Complement Alternat Med Anti-inflammatory Mechanism of Ruzu Bitters on Diet-Induced Nonalcoholic Fatty Liver Disease in Male Wistar Rats. |