Rat Insulin ELISA Kit
Validation Data Gallery
Product Information
KE20008 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Insulin ELISA kit is to be used to detect and quantify protein levels of endogenous Insulin. The assay recognizes Rat Insulin. An antibody specific for Insulin has been pre-coated onto the microwells. The Insulin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for Insulin is added to detect the captured Insulin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Rat Insulin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.2 ng/mL |
| Range | 0.31-20 ng/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24505 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 107% | 90%-124% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 0.5 | 0.04 | 6.6 |
| 2 | 20 | 2.4 | 0.18 | 7.4 |
| 3 | 20 | 9.2 | 0.68 | 7.4 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 0.6 | 0.06 | 9.6 |
| 2 | 24 | 2.4 | 0.15 | 6.0 |
| 3 | 24 | 9.4 | 0.71 | 7.5 |
Background Information
Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in insulin results in type 1 diabetes mellitus.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ins1, insulin 1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Phytomedicine Moringa oleifera Lam. seed extract protects kidney function in rats with diabetic nephropathy by increasing GSK-3β activity and activating the Nrf2/HO-1 pathway. | ||
Front Microbiol Changes in the Gut Metabolic Profile of Gestational Diabetes Mellitus Rats Following Probiotic Supplementation. | ||
J Agric Food Chem Ameliorating Effect of the Total Flavonoids of Morus nigra L. on Prediabetic Mice Based on Regulation of Inflammation and Insulin Sensitization | ||
Endokrynol Pol Neu-P11, a novel melatonin receptor agonist, could improve the features of type-2 diabetes mellitus in rats. | ||
Chin J Nat Med Eucommia lignans alleviate the progression of diabetic nephropathy through mediating the AR/Nrf2/HO-1/AMPK axis in vivo and in vitro | ||
ACS Nano Acidic Nanoparticles Restore Lysosomal Acidification and Rescue Metabolic Dysfunction in Pancreatic β-Cells under Lipotoxic Conditions |