Rat Total MMP-9 ELISA Kit
Sensitivity
14.9 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Rat
Validation Data Gallery
Product Information
KE20006 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Rat total MMP9 ELISA kit is to be used to detect and quantify protein levels of endogenous rat total MMP9. The assay recognizes rat total MMP9. An antibody specific for rat total MMP9 has been pre-coated onto the microwells. The rat total MMP9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat total MMP9 is added to detect the captured rat total MMP9 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat Total MMP-9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 14.9 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 81687 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 106% | 98%-117% |
| Cell culture supernatants | 102% | 96%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1507.7 | 73.2 | 4.9 |
| 2 | 20 | 335.2 | 21.9 | 6.5 |
| 3 | 20 | 77.6 | 4.7 | 6.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1456.6 | 47.1 | 3.2 |
| 2 | 24 | 356.1 | 20.2 | 5.7 |
| 3 | 24 | 84.1 | 6.8 | 8.1 |
Background Information
MMP9 (matrix metallopeptidase 9), also named as Gelatinase B, is a member of matrix metalloproteinase (MMP) family. The MMP family of enzymes is comprised of critically important extracellular matrix remodeling proteases whose activity has been implicated in normal embryogenesis, tissue remodelling and many diseases such as arthritis, cancer, periodontitis, glomerulonephritis, encephalomyelitis, atherosclerosis and tissue ulceration. MMP9 is produced by a variety of normal and transformed cells including neutrophils, monocytes, macrophages, astrocytes, fbroblasts, osteoclasts and so on. Transgenic mouse models report that MMP9 contributes to skin carcinogenesis, suppresses development of experimental abdominal aortic aneurysms, and triggers the angiogenic switch during carcinogenesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | matrix metallopeptidase 9, Mmp9, MMP-9 |