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验证数据展示

  • WB analysis of human lung using 66227-1-Ig (same clone as 66227-1-PBS)

    WB analysis of human lung using 66227-1-Ig (same clone as 66227-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 66227-1-Ig (S100A10 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    IHC staining of human colon cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A10 antibody included
  • IHC staining of human colon cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    IHC staining of human colon cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A10 antibody included
  • IF Staining of A431 using 66227-1-Ig (same clone as 66227-1-PBS)

    IF Staining of A431 using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using S100A10 antibody (66227-1-Ig, Clone: 1B3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • IF Staining of Saos-2 using 66227-1-Ig (same clone as 66227-1-PBS)

    IF Staining of Saos-2 using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using S100A10 antibody (66227-1-Ig, Clone: 1B3F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    IHC staining of human stomach cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A10 antibody included
  • IHC staining of human liver cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    IHC staining of human liver cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A10 antibody included
  • IHC staining of human liver cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    IHC staining of human liver cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A10 antibody included
  • IHC staining of human cervical cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    IHC staining of human cervical cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A10 antibody included
  • IHC staining of human cervical cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    IHC staining of human cervical cancer using 66227-1-Ig (same clone as 66227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A10 antibody included

S100A10 Monoclonal antibody, PBS Only

S100A10 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66227-1-PBS

产品说明书

CloneNo. 1B3F9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy S100A10 Ready-To-Use IHC Kit. S100A10 primary antibody included.

产品信息

66227-1-PBS targets S100A10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 S100A10 fusion protein Ag23596 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 S100 calcium binding protein A10
别名 42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10
计算分子量 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC015973
基因名称 S100A10
Gene ID (NCBI) 6281
RRIDAB_2881618
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP60903
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.