验证数据展示
经过测试的应用
Positive WB detected in | THP-1 cells, A549 cells, COLO 320 cells, HL-60 cells, MCF-7 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human stomach cancer tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | THP-1 cells |
Positive FC (Intra) detected in | RAW 264.7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15792-1-AP targets S100A8 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse, rat |
免疫原 | S100A8 fusion protein Ag8500 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | S100 calcium binding protein A8 |
别名 | CGLA, CFAG, Calprotectin L1L subunit, Calgranulin-A, Calgranulin A |
计算分子量 | 93 aa, 11 kDa |
观测分子量 | 11 kDa |
GenBank蛋白编号 | BC005928 |
基因名称 | S100A8 |
Gene ID (NCBI) | 6279 |
RRID | AB_10666315 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P05109 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9(16216873).
实验方案
Product Specific Protocols | |
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WB protocol for S100A8 antibody 15792-1-AP | Download protocol |
IHC protocol for S100A8 antibody 15792-1-AP | Download protocol |
IF protocol for S100A8 antibody 15792-1-AP | Download protocol |
IP protocol for S100A8 antibody 15792-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Rep Med Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors | ||
Nat Commun S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy. | ||
Mol Ther Bulk and single-cell transcriptome profiling reveal the metabolic heterogeneity in human breast cancers.
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Acta Physiol (Oxf) Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion. |