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S100A8 Polyclonal antibody

S100A8 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 15792-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

CGLA, CFAG, Calprotectin L1L subunit, Calgranulin-A, Calgranulin A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inTHP-1 cells, A549 cells, COLO 320 cells, HL-60 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman stomach cancer tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells
Positive FC (Intra) detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy S100A8 Ready-To-Use IHC Kit. S100A8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15792-1-AP targets S100A8 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 S100A8 fusion protein Ag8500 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S100 calcium binding protein A8
别名 CGLA, CFAG, Calprotectin L1L subunit, Calgranulin-A, Calgranulin A
计算分子量 93 aa, 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC005928
基因名称 S100A8
Gene ID (NCBI) 6279
RRIDAB_10666315
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05109
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9(16216873).

实验方案

Product Specific Protocols
WB protocol for S100A8 antibody 15792-1-APDownload protocol
IHC protocol for S100A8 antibody 15792-1-APDownload protocol
IF protocol for S100A8 antibody 15792-1-APDownload protocol
IP protocol for S100A8 antibody 15792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Metab

QDPR deficiency drives immune suppression in pancreatic cancer

Authors - Ji Liu
humanWB

Mol Cell

Hexokinase 1 cellular localization regulates the metabolic fate of glucose.

Authors - Adam De Jesus
humanWB

Cell Rep Med

Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors

Authors - Yiting Wang
mouseWB,IHC

Nat Commun

S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy.

Authors - Jianjie Li
humanWB

Mol Ther

Bulk and single-cell transcriptome profiling reveal the metabolic heterogeneity in human breast cancers.

Authors - Tian-Jian Yu
  • KD Validated
ratWB

Acta Physiol (Oxf)

Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.

Authors - Li Zhang
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