验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue, human blood, human heart tissue, MCF-7 cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human breast cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14226-1-AP targets S100A9 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | S100A9 fusion protein Ag5465 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | S100 calcium binding protein A9 |
| Synonyms | 60B8AG, CAGB, Calgranulin B, Calprotectin L1H subunit, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP 14, MRP14, NIF, P14, Protein S100 A9, S100A9 |
| Calculated Molecular Weight | 13 kDa |
| Observed Molecular Weight | 14 kDa, 28 kDa |
| GenBank Accession Number | BC047681 |
| Gene Symbol | S100A9 |
| Gene ID (NCBI) | 6280 |
| RRID | AB_2254232 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06702 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for S100A9 antibody 14226-1-AP | Download protocol |
| IHC protocol for S100A9 antibody 14226-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Cancer S100 calcium-binding protein A9 from tumor-associated macrophage enhances cancer stem cell-like properties of hepatocellular carcinoma. | ||
Cell Biosci TRPM8 deficiency attenuates liver fibrosis through S100A9-HNF4α signaling.
| ||
Int J Med Sci Single-cell RNA Sequencing Uncovered the Involvement of an Endothelial Subset in Neutrophil Recruitment in Chemically Induced Rat Pulmonary Inflammation. | ||
J Oncol Downregulation of S100A9 Reverses Cisplatin-Resistance and Inhibits Proliferation and Migration in Hypopharyngeal Carcinoma
| ||
Histol Histopathol Histopathological assessment of calcification and inflammation of calcific aortic valves from patients with and without diabetes mellitus. |
