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  • Featured Product
  • KD/KO Validated

S100A9 Polyclonal antibody

S100A9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 26992-1-AP

Print datasheet

Synonyms

L1AG, CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B

验证数据展示

  • WB analysis using 26992-1-AP

    WB analysis using 26992-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26992-1-AP (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 26992-1-AP

    WB analysis of MCF-7 using 26992-1-AP

    WB result of S100A9 antibody (26992-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A9 transfected MCF-7 cells.

  • IHC staining of human breast cancer using 26992-1-AP

    IHC staining of human breast cancer using 26992-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A9 antibody included
  • IHC staining of mouse spleen using 26992-1-AP

    IHC staining of mouse spleen using 26992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A9 antibody included
  • IHC staining of human liver cancer using 26992-1-AP

    IHC staining of human liver cancer using 26992-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A9 antibody included
  • IHC staining of human breast cancer using 26992-1-AP

    IHC staining of human breast cancer using 26992-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A9 antibody included
  • IF Staining of HeLa using 26992-1-AP

    IF Staining of HeLa using 26992-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using S100A9 antibody (26992-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A549 using 26992-1-AP

    IF Staining of A549 using 26992-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using S100A9 antibody (26992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 26992-1-AP

    IF Staining of HepG2 using 26992-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S100A9 antibody (26992-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of MCF-7 using 26992-1-AP

    IP experiment of MCF-7 using 26992-1-AP

    IP result of anti-S100A9 (IP:26992-1-AP, 4ug; Detection:26992-1-AP 1:3000) with MCF-7 cells lysate 1600 ug.

经过测试的应用

Positive WB detected inHL-60 cells, MCF-7 cells, THP-1 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, mouse spleen tissue, human tonsillitis tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A549 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy S100A9 Ready-To-Use IHC Kit. S100A9 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26992-1-AP targets S100A9 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen S100A9 fusion protein Ag25764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name S100 calcium binding protein A9
Synonyms L1AG, CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC047681
Gene Symbol S100A9
Gene ID (NCBI) 6280
RRIDAB_2880716
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06702
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

实验方案

Product Specific Protocols
WB protocol for S100A9 antibody 26992-1-APDownload protocol
IHC protocol for S100A9 antibody 26992-1-APDownload protocol
IF protocol for S100A9 antibody 26992-1-APDownload protocol
IP protocol for S100A9 antibody 26992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Authors - Shaojun Zhang
View Article
humanWB

Cell Rep Med

Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors

Authors - Yiting Wang
View Article
mouseFC

Sci Adv

Gut microbiota from patients with arteriosclerotic CSVD induces higher IL-17A production in neutrophils via activating RORγt.

Authors - Wei Cai
View Article
humanCoIP

Theranostics

Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation.

Authors - Hong Xiang
  • KD Validated
View Article
mouseWB,IHC

Theranostics

Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells.

Authors - Genyu Liu
View Article
ratWB

Acta Physiol (Oxf)

Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.

Authors - Li Zhang
View Article