验证数据展示

WB analysis using 26992-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26992-1-AP (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 26992-1-AP
WB result of S100A9 antibody (26992-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A9 transfected MCF-7 cells.
IHC staining of human breast cancer using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with S100A9 antibody included
IHC staining of mouse spleen using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with S100A9 antibody included
IHC staining of human liver cancer using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26992-1-AP (S100A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with S100A9 antibody included
IHC staining of human breast cancer using 26992-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26992-1-AP (S100A9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with S100A9 antibody included
IF Staining of HeLa using 26992-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using S100A9 antibody (26992-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A549 using 26992-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using S100A9 antibody (26992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 26992-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S100A9 antibody (26992-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP experiment of MCF-7 using 26992-1-AP
IP result of anti-S100A9 (IP:26992-1-AP, 4ug; Detection:26992-1-AP 1:3000) with MCF-7 cells lysate 1600 ug.

Featured Product
KD/KO验证

S100A9 Polyclonal antibody

S100A9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 26992-1-AP

产品说明书

引用文献(56)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

L1AG, CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite®594

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (56)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HL-60 cells, MCF-7 cells, THP-1 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue, mouse spleen tissue, human tonsillitis tissue, human oesophagus cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, A549 cells, HepG2 cells

Planning an IHC experiment? We recommend our IHCeasy S100A9 Ready-To-Use IHC Kit. S100A9 primary antibody included.

Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 32 publications below
IHC	See 13 publications below
IF	See 15 publications below
IP	See 3 publications below
FC	See 2 publications below
CoIP	See 2 publications below

产品信息

26992-1-AP targets S100A9 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat
免疫原	S100A9 fusion protein Ag25764 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	S100 calcium binding protein A9
别名	L1AG, CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B
计算分子量	13 kDa
观测分子量	14 kDa
GenBank蛋白编号	BC047681
基因名称	S100A9
Gene ID (NCBI)	6280
RRID	AB_2880716
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P06702
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

实验方案

Product Specific Protocols
WB protocol for S100A9 antibody 26992-1-AP	Download protocol
IHC protocol for S100A9 antibody 26992-1-AP	Download protocol
IF protocol for S100A9 antibody 26992-1-AP	Download protocol
IP protocol for S100A9 antibody 26992-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Authors - Shaojun Zhang View Article
human	WB	Cell Rep Med Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors Authors - Yiting Wang View Article
mouse	FC	Sci Adv Gut microbiota from patients with arteriosclerotic CSVD induces higher IL-17A production in neutrophils via activating RORγt. Authors - Wei Cai View Article
human	CoIP	Theranostics Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation. Authors - Hong Xiang KD Validated View Article
mouse	WB,IHC	Theranostics Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells. Authors - Genyu Liu View Article
rat	WB	Acta Physiol (Oxf) Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion. Authors - Li Zhang View Article

验证数据展示

WB analysis using 26992-1-AP

WB analysis of MCF-7 using 26992-1-AP

IHC staining of human breast cancer using 26992-1-AP

IHC staining of mouse spleen using 26992-1-AP

IHC staining of human liver cancer using 26992-1-AP

IHC staining of human breast cancer using 26992-1-AP

IF Staining of HeLa using 26992-1-AP

IF Staining of A549 using 26992-1-AP

IF Staining of HepG2 using 26992-1-AP

IP experiment of MCF-7 using 26992-1-AP

S100A9 Polyclonal antibody

S100A9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines a class of antibodies to fight solid tumors

Gut microbiota from patients with arteriosclerotic CSVD induces higher IL-17A production in neutrophils via activating RORγt.

Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation.

Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells.

Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.