S100 Beta Polyclonal antibody

S100 Beta Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15146-1-AP

Print datasheet

Synonyms

NEF, Protein S100 B, S 100 protein beta chain, S 100 protein subunit beta, S100, S100 Beta, S100 calcium binding protein B, S100B, S100beta



经过测试的应用

Positive WB detected inA375 cells, mouse brain tissue, human testis tissue, human brain tissue, U-251 cells, C6 cells
Positive IHC detected inhuman malignant melanoma tissue, human gliomas tissue, mouse brain tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, human astrocytes, A375 cells, mouse cerebellum tissue, human malignant melanoma tissue
Positive FC detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy S100B Ready-To-Use IHC Kit. S100B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15146-1-AP targets S100 Beta in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited ApplicationsFC, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen S100 Beta fusion protein Ag7440 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name S100 calcium binding protein B
Synonyms NEF, Protein S100 B, S 100 protein beta chain, S 100 protein subunit beta, S100, S100 Beta, S100 calcium binding protein B, S100B, S100beta
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC001766
Gene Symbol S100B
Gene ID (NCBI) 6285
RRIDAB_2254244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04271
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100B belongs to the EF-hand calcium binding proteins and is found primarily in astrocytes in the central nervous system (CNS). S100B has a variety of functions, including calcium homeostasis, cell proliferation, differentiation, migration, and survival, as well as neurite outgrowth and regeneration.

 1. What is the molecular weight of S100B?

S100B protein is composed of non-covalently linked homodimers of 11 kDa size.

2. What is the subcellular localization of S100B?

S100B localizes to the nucleus and cytoplasm, associating with intracellular membranes, the centrosomes, microtubules, and type III intermediate filaments (PMID: 19110011). Additionally, it can be released from astrocytes into the extracellular space and can enter the bloodstream.

3. What is the expression pattern of S100B?

S100B is predominantly expressed in astrocytes and maturing oligodendrocytes but is also present in other cell types such as kidney epithelial cells, neural progenitor cells, pituicytes, ependymocytes, chondrocytes, adipocytes, melanocytes, Langerhans cells, dendritic cells, certain lymphocyte subpopulations, skeletal myofibers, myoblasts, and muscle satellite cells (PMID: 19110011). S100B is a commonly used marker of Schwann cells and reactive astrocytes in ICC, IHC, and WB applications.

4. What is the diagnostic use of S100B in the clinic?

S100B is naturally secreted by astrocytes into the extracellular space and low amounts of S100B can pass through the brain-blood barrier and enter the bloodstream. Elevated levels of S100B in the serum are observed in patients with traumatic head injuries, as well as in patients suffering from neurodegenerative diseases (PMID: 30144068). This increase in S100B levels is attributed to the elevated secretion of S100B protein from astrocytes as part of the physiological response to the injury, as well as to the physical damage of astrocytes and increased blood-brain barrier permeability.


实验方案

Product Specific Protocols
WB protocol for S100 Beta antibody 15146-1-APDownload protocol
IHC protocol for S100 Beta antibody 15146-1-APDownload protocol
IF protocol for S100 Beta antibody 15146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nature

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Authors - Hao Qian
mouseIHC

Nature

Regulation of intestinal immunity and tissue repair by enteric glia

Authors - Fränze Progatzky
mouseIHC

Cell

Mechanoreceptor synapses in the brainstem shape the central representation of touch.

Authors - Brendan P Lehnert
mouseIF

Nat Commun

Alk1 acts in non-endothelial VE-cadherin+ perineurial cells to maintain nerve branching during hair homeostasis

Authors - Gopal Chovatiya
mouseIF

Neuron

γ-Protocadherins control synapse formation and peripheral branching of touch sensory neurons

Authors - Shan Meltzer
humanIF

Small Methods

Polydopamine-Decorated PLCL Conduit to Induce Synergetic Effect of Electrical Stimulation and Topological Morphology for Peripheral Nerve Regeneration

Authors - Shunyi Lu