S100B Monoclonal antibody

S100B Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66616-1-Ig

产品说明书

CloneNo. 1G10F4

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, IF-P, ELISA and More (1)

S100 beta, 1G10F4, Protein S100 B, Protein S100-B, S100 calcium-binding protein B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inrabbit brain tissue, rat brain tissue, rat cerebellum tissue, rat spinal cord tissue
Positive IHC detected inrat brain tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Planning an IHC experiment? We recommend our IHCeasy S100B Ready-To-Use IHC Kit. S100B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66616-1-Ig targets S100B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, rat
文献引用反应性human, rat
免疫原 S100B fusion protein Ag7440 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 S100 calcium binding protein B
别名 S100 beta, 1G10F4, Protein S100 B, Protein S100-B, S100 calcium-binding protein B
计算分子量 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC001766
基因名称 S100 Beta
Gene ID (NCBI) 6285
RRIDAB_2881976
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP04271
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.

实验方案

Product Specific Protocols
WB protocol for S100B antibody 66616-1-IgDownload protocol
IHC protocol for S100B antibody 66616-1-IgDownload protocol
IF protocol for S100B antibody 66616-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Acta Neuropathol Commun

Repetitive mild TBI causes pTau aggregation in nigra without altering preexisting fibril induced Parkinson’s-like pathology burden

Authors - Vedad Delic
ratIHC

ACS Omega

Platelet-Rich Plasma Gel-Loaded Collagen/Chitosan Composite Film Accelerated Rat Sciatic Nerve Injury Repair

Authors - Bo Yuan
ratIF

Front Neurosci

Nogo-C Inhibits Peripheral Nerve Regeneration by Regulating Schwann Cell Apoptosis and Dedifferentiation.

Authors - Bo Jia
humanIF

Transl Oncol

High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma.

Authors - Zhigang Wang
ratIF

Exp Neurol

Motor neurons transplantation alleviates neurofibrogenesis during chronic degeneration by reversibly regulating Schwann cells epithelial-mesenchymal transition

Authors - Zhaoyang Wu
IF,WB

Neurochem Res

Resistin-Inhibited Neural Stem Cell-Derived Astrocyte Differentiation Contributes to Permeability Destruction of the Blood-Brain Barrier.

Authors - Liu Xiaoying
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