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Species Reactivity
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验证数据展示

  • WB analysis using 66886-1-Ig (same clone as 66886-1-PBS)

    WB analysis using 66886-1-Ig (same clone as 66886-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

  • WB analysis using 66886-1-Ig (same clone as 66886-1-PBS)

    WB analysis using 66886-1-Ig (same clone as 66886-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66886-1-Ig (same clone as 66886-1-PBS)

    IHC staining of human placenta using 66886-1-Ig (same clone as 66886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPS6 antibody included
  • IHC staining of human placenta using 66886-1-Ig (same clone as 66886-1-PBS)

    IHC staining of human placenta using 66886-1-Ig (same clone as 66886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPS6 antibody included
  • IHC staining of mouse pancreas using 66886-1-Ig (same clone as 66886-1-PBS)

    IHC staining of mouse pancreas using 66886-1-Ig (same clone as 66886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPS6 antibody included
  • IHC staining of rat stomach using 66886-1-Ig (same clone as 66886-1-PBS)

    IHC staining of rat stomach using 66886-1-Ig (same clone as 66886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPS6 antibody included
  • IF Staining of HepG2 using 66886-1-Ig (same clone as 66886-1-PBS)

    IF Staining of HepG2 using 66886-1-Ig (same clone as 66886-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S6 Ribosomal protein antibody (66886-1-Ig, Clone: 1C3E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66886-1-Ig (same clone as 66886-1-PBS)

    FC experiment of HepG2 using 66886-1-Ig (same clone as 66886-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human S6 Ribosomal protein (66886-1-Ig, Clone:1C3E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

S6 Ribosomal protein Monoclonal antibody, PBS Only

S6 Ribosomal protein Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66886-1-PBS

产品说明书

CloneNo. 1C3E10
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

RPS6, S6, 1C3E10, 40S ribosomal protein S6, OK/SW-cl.2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.

发表文章中的应用

WBSee 1 publications below

产品信息

66886-1-PBS targets S6 Ribosomal protein in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag6599

Product name: Recombinant human RPS6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-249 aa of BC000524

Sequence: MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein S6
别名 RPS6, S6, 1C3E10, 40S ribosomal protein S6, OK/SW-cl.2
计算分子量 29 kDa
观测分子量 29-32 kDa
GenBank蛋白编号BC000524
基因名称 RPS6
Gene ID (NCBI) 6194
RRIDAB_2882218
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62753
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

发表文章

SpeciesApplicationTitle
humanWB

Transl Oncol

Next-generation sequencing in early-stage multiple primary lung cancer: The prognostic significance of genomic accumulation status and BCL2L11del

Authors - Mu-Ting Wang
View Article