验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells, A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-12586 targets SAMHD1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | SAMHD1 fusion protein Ag3287 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SAM domain and HD domain 1 |
| 别名 | DCIP, HDDC1, Monocyte protein 5, MOP 5, MOP5, SAM domain and HD domain 1, SAMHD1, SBBI88 |
| 计算分子量 | 626 aa, 72 kDa |
| 观测分子量 | 64-72 kDa |
| GenBank蛋白编号 | BC036450 |
| 基因名称 | SAMHD1 |
| Gene ID (NCBI) | 25939 |
| RRID | AB_2919785 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y3Z3 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with a presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 SAMHD1 antibody CL594-12586 | Download protocol |
| FC protocol for CL594 SAMHD1 antibody CL594-12586 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |