- Featured Product
- KD/KO Validated
SAMHD1 Polyclonal antibody
SAMHD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, K-562 cells, mouse heart tissue, human heart tissue, L02 cells, human brain tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12586-1-AP targets SAMHD1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, ChIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Immunogen | SAMHD1 fusion protein Ag3287 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SAM domain and HD domain 1 |
| Synonyms | HDDC1, EC:3.1.5.-, dNTPase, Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, Dendritic cell-derived IFNG-induced protein |
| Calculated Molecular Weight | 626 aa, 72 kDa |
| Observed Molecular Weight | 64-72 kDa |
| GenBank Accession Number | BC036450 |
| Gene Symbol | SAMHD1 |
| Gene ID (NCBI) | 25939 |
| RRID | AB_2183496 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3Z3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SAMHD1 antibody 12586-1-AP | Download protocol |
| IHC protocol for SAMHD1 antibody 12586-1-AP | Download protocol |
| IF protocol for SAMHD1 antibody 12586-1-AP | Download protocol |
| IP protocol for SAMHD1 antibody 12586-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Genet Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response. | ||
Nat Microbiol Human cytomegalovirus overcomes SAMHD1 restriction in macrophages via pUL97. | ||
Immunity Tissue Myeloid Cells in SIV-Infected Primates Acquire Viral DNA through Phagocytosis of Infected T Cells. |
