• Featured Product
  • KD/KO Validated

SAR1A Polyclonal antibody

SAR1A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 22291-1-AP

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Synonyms

COPII associated small GTPase, GTP binding protein SAR1a, masra2, SAR1, SAR1 homolog A (S. cerevisiae), SAR1A, Sara, SARA1



经过测试的应用

Positive WB detected inA431 cells, HT-1080 cells, HepG2 cells, mouse kidney tissue, HEK-293T cells, HeLa cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SAR1A Ready-To-Use IHC Kit. SAR1A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22291-1-AP targets SAR1A in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsCoIP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SAR1 homolog A (S. cerevisiae)
Synonyms COPII associated small GTPase, GTP binding protein SAR1a, masra2, SAR1, SAR1 homolog A (S. cerevisiae), SAR1A, Sara, SARA1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 23-25 kDa
GenBank Accession NumberNM_020150
Gene Symbol SAR1A
Gene ID (NCBI) 56681
RRIDAB_2879062
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR31
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). The antibody is specific to SAR1A.

实验方案

Product Specific Protocols
WB protocol for SAR1A antibody 22291-1-APDownload protocol
IHC protocol for SAR1A antibody 22291-1-APDownload protocol
IF protocol for SAR1A antibody 22291-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
  • KO Validated
humanCoIP

J Cell Sci

A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.

Authors - Jiaxin Sun
  • KD Validated
ratWB

Ann Transl Med

Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage.

Authors - Kun Wang
humanWB

Epigenomics

Methylation of TMEM176A, a key ERK signaling regulator, is a novel synthetic lethality marker of ATM inhibitors in human lung cancer.

Authors - Hongxia Li