验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HT-1080 cells, HepG2 cells, mouse kidney tissue, HEK-293T cells, HeLa cells |
| Positive IHC detected in | mouse skeletal muscle tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A431 cells |
Planning an IHC experiment? We recommend our IHCeasy SAR1A Ready-To-Use IHC Kit. SAR1A primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| CoIP | See 1 publications below |
产品信息
22291-1-AP targets SAR1A in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SAR1 homolog A (S. cerevisiae) |
| 别名 | SARA, SAR1, GTP binding protein SAR1a, EC:3.6.5.2, COPII-associated small GTPase |
| 计算分子量 | 22 kDa |
| 观测分子量 | 23-25 kDa |
| GenBank蛋白编号 | NM_020150 |
| 基因名称 | SAR1A |
| Gene ID (NCBI) | 56681 |
| RRID | AB_2879062 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NR31 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). The antibody is specific to SAR1A.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SAR1A antibody 22291-1-AP | Download protocol |
| IHC protocol for SAR1A antibody 22291-1-AP | Download protocol |
| IF protocol for SAR1A antibody 22291-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Sci A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.
| ||
Ann Transl Med Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage. | ||
Epigenomics Methylation of TMEM176A, a key ERK signaling regulator, is a novel synthetic lethality marker of ATM inhibitors in human lung cancer. | ||
Cell Death Differ C9orf72 controls hepatic lipid metabolism by regulating SREBP1 transport | ||
J Cell Biol Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells |
