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Virus SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal antibody, PBS Only (Capture)

SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal Antibody for WB, ELISA, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

virus

Applications

WB, ELISA, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B3C3

Cat no : 67666-1-PBS

Print datasheet

Synonyms

nucleocapsid phosphoprotein, sars cov 2, 1B3C3

验证数据展示

  • ELISA experiment of Recombinant protein using 67666-1-Ig (same clone as 67666-1-PBS)

    ELISA experiment of Recombinant protein using 67666-1-Ig (same clone as 67666-1-PBS)

    Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 67666-1-Ig and 67666-2-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 67666-1-Ig (same clone as 67666-1-PBS)

    ELISA experiment of Recombinant protein using 67666-1-Ig (same clone as 67666-1-PBS)

    Sandwich ELISA was carried out by coating 67666-1-Ig at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated clone 67666-2-Ig was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

  • WB analysis using 67666-1-Ig (same clone as 67666-1-PBS)

    WB analysis using 67666-1-Ig (same clone as 67666-1-PBS)

    E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 67666-1-Ig and 67666-2-Ig at various work concentration. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50061-1

    Sandwich ELISA standard curve of MP50061-1

    Sandwich ELISA standard curve of MP50061-1, Virus 2019-nCOV nucleocapsid phosphoprotein Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67666-1-PBS. Detection antibody: HRP-conjugated 67666-2-PBS. Standard: Ag30676. Range: 0.5-20 ng/mL.

产品信息

67666-1-PBS targets SARS-CoV-2 Nucleocapsid Phosphoprotein as part of a matched antibody pair:

MP50061-1: 67666-1-PBS capture and 67666-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, ELISA, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity virus
Immunogen SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name COVID-19 N Protein
Synonyms nucleocapsid phosphoprotein, sars cov 2, 1B3C3
GenBank Accession NumberNC_045512
Gene Symbol COVID-19 N Protein
Gene ID (NCBI) 43740575
RRIDAB_2882862
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387).67666-1-Ig can be used as capture antibody. 67666-2-Ig can be used as detection antibody.