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SARS-CoV-2 Spike Recombinant antibody

SARS-CoV-2 Spike Recombinant Antibody for WB, ELISA

Host / Isotype

Human / IgG1

Reactivity

Virus

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

AM004414

Cat no : 91345-PTG

Print datasheet

Synonyms

SARS-CoV-2 Spike

验证数据展示

  • WB analysis of HEK293 cells using 91345-PTG

    WB analysis of HEK293 cells using 91345-PTG

    SARS-CoV-2 Spike Antibody (AM004414) tested by Western blot.Lysates from HEK293 cells transfected (Lane 1 and 3) or untransfected (Lane 2) was probed with 0.5ug/mL of rAb (Lane 1 & 2) or an anti-flag Ab (Lane 3).Lane 1: transfected flag-tagged SARS-CoV-2 S1 subunitLane 2 untransfectedLane 3: transfected flag-tagged SARS-CoV-2 S1 subunit. Data provided by Active Motif(R). .

  • ELISA experiment of SARS-CoV-2 Spike RBD protein using 91345-PTG

    ELISA experiment of SARS-CoV-2 Spike RBD protein using 91345-PTG

    SARS-CoV-2 Spike Antibody (clone AM004414) tested by ELISA.SARS-CoV-2 Spike RBD protein was coated onto microtiter plates at 0.5 µg/mL and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (clone AM009105). Bound antibodies were detected with anti-human IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm. Data provided by Active Motif®. .

经过测试的应用

Positive WB detected inHEK293 cells
Positive ELISA detected inSARS-CoV-2 Spike RBD protein

推荐稀释比

Sample dependent. To be determined by the end user.

Note: Proteintech is partnering with Active Motif to bring these cutting-edge, patient derived antibodies to our customers during this important time for COVID-19 research.

产品信息

91345-PTG targets SARS-CoV-2 Spike in WB, ELISA applications and shows reactivity with Virus samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Virus
Immunogen Virus 种属同源性预测
Host / Isotype Human / IgG1
Class Recombinant
Type Antibody
Full Name SARS-CoV-2 Spike Protein
Synonyms SARS-CoV-2 Spike
Calculated Molecular Weight 141 kDa
GenBank Accession NumberNC_045512
Gene Symbol SARS-COV-2
Gene ID (NCBI) 43740568
RRIDAB_2882949
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A Chromatography
UNIPROT IDP0DTC2
Storage Buffer 140 mM Hepes, pH 7.5, 70 mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies. This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells.