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验证数据展示

  • WB analysis of HEK-293 using 91351-PTG

    WB analysis of HEK-293 using 91351-PTG

    SARS-CoV-2 Spike Antibody (AM006415) tested by Western blot.Lysates from HEK293 cells transfected (Lane 1 and 3) or untransfected (Lane 2) was probed with 0.5ug/mL of rAb (Lane 1 & 2) or an anti-flag Ab (Lane 3).Lane 1: transfected flag-tagged SARS-CoV-2 S1 subunitLane 2 untransfectedLane 3: transfected flag-tagged SARS-CoV-2 S1 subunit. Data provided by Active Motif®. .

  • ELISA experiment of SARS-CoV-2 Spike RBD protein using 91351-PTG

    ELISA experiment of SARS-CoV-2 Spike RBD protein using 91351-PTG

    SARS-CoV-2 Spike Antibody (clone AM006415) tested by ELISA.SARS-CoV-2 Spike RBD protein was coated onto microtiter plates at 0.5 µg/mL and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (clone AM009105). Bound antibodies were detected with anti-human IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm. Data provided by Active Motif®. .

SARS-CoV-2 Spike Recombinant antibody

SARS-CoV-2 Spike Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 91351-PTG

产品说明书

CloneNo. AM006415

宿主/亚型

Human / IgG1

种属反应性

Virus

应用

WB, ELISA

别名

SARS-CoV-2, SARS-CoV-2 Spike, Spike

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive ELISA detected inSARS-CoV-2 Spike RBD protein

推荐稀释比

Sample dependent. To be determined by the end user.

Note: Proteintech is partnering with Active Motif to bring these cutting-edge, patient derived antibodies to our customers during this important time for COVID-19 research.

产品信息

91351-PTG targets SARS-CoV-2 Spike in WB, ELISA applications and shows reactivity with Virus samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Virus
免疫原 Virus 种属同源性预测
宿主/亚型 Human / IgG1
抗体类别 Recombinant
产品类型 Antibody
全称 SARS-CoV-2 Spike Protein
别名 SARS-CoV-2, SARS-CoV-2 Spike, Spike
计算分子量 141 kDa
GenBank蛋白编号NC_045512
基因名称 SARS-COV-2
Gene ID (NCBI) 43740568
RRIDAB_2882952
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A Chromatography
UNIPROT IDP0DTC2
储存缓冲液 140 mM Hepes, pH 7.5, 70 mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies. This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells.