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SCLT1 Polyclonal antibody

SCLT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14875-1-AP

Print datasheet

Synonyms

Sodium channel-associated protein 1, SAP1, hCAP 1A, CAP1A

验证数据展示

  • IHC staining of human colon cancer using 14875-1-AP

    IHC staining of human colon cancer using 14875-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14875-1-AP (SCLT1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 14875-1-AP

    IHC staining of human colon cancer using 14875-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14875-1-AP (SCLT1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 14875-1-AP

    WB analysis using 14875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14875-1-AP (SCLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of human placenta using 14875-1-AP

    IP experiment of human placenta using 14875-1-AP

    IP result of anti-SCLT1 (IP:14875-1-AP, 4ug; Detection:14875-1-AP 1:1000) with human placenta tissue lysate 4000ug.

  • WB analysis using 14875-1-AP

    WB analysis using 14875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14875-1-AP (SCLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14875-1-AP

    WB analysis using 14875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14875-1-AP (SCLT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 14875-1-AP

    IF Staining of hTERT-RPE1 using 14875-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using SCLT1 antibody (14875-1-AP) at dilution of 1:600 and CoraLite® Plus 647-conjugated AffiniPure F(ab, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green), Centrin 1 antibody (12794-1-AP, orange).

  • IF Staining of hTERT-RPE1 using 14875-1-AP

    IF Staining of hTERT-RPE1 using 14875-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using SCLT1 antibody (14875-1-AP) at dilution of 1:600 and CoraLite® Plus 647-conjugated AffiniPure F(ab, CEP192 antibody (18832-1-AP, orange), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • IF Staining of hTERT-RPE1 using 14875-1-AP

    IF Staining of hTERT-RPE1 using 14875-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using SCLT1 antibody (14875-1-AP) at dilution of 1:600 and CoraLite® Plus 647-conjugated AffiniPure F(ab, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green), CEP250/CNAP1 antibody (14498-1-AP, orange).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, COLO 320 cells, rat brain tissue, HeLa cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14875-1-AP targets SCLT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SCLT1 fusion protein Ag6646 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sodium channel and clathrin linker 1
Synonyms Sodium channel-associated protein 1, SAP1, hCAP 1A, CAP1A
Calculated Molecular Weight 81 kDa
Observed Molecular Weight36 kDa,75-80 kDa
GenBank Accession NumberBC064428
Gene Symbol SCLT1
Gene ID (NCBI) 132320
RRIDAB_2183691
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NL6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCLT1 antibody 14875-1-APDownload protocol
IHC protocol for SCLT1 antibody 14875-1-APDownload protocol
IF protocol for SCLT1 antibody 14875-1-APDownload protocol
IP protocol for SCLT1 antibody 14875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

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