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  • Featured Product
  • KD/KO Validated

SDHB Polyclonal antibody

SDHB Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10620-1-AP

Print datasheet

Synonyms

SDH 1, SDH, PGL4, PGL 4, Iron-sulfur subunit of complex II

验证数据展示

  • IHC staining of human heart using 10620-1-AP

    IHC staining of human heart using 10620-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 10620-1-AP

    IHC staining of human heart using 10620-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of HepG2 using 10620-1-AP

    WB analysis of HepG2 using 10620-1-AP

    WB result of SDHB antibody (10620-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SDHB transfected HepG2 cells.

  • IP experiment of HEK-293 using 10620-1-AP

    IP experiment of HEK-293 using 10620-1-AP

    IP result of anti-SDHB (IP:10620-1-AP, 4ug; Detection:10620-1-AP 1:5000) with HEK-293 cells lysate 1360 ug.

  • WB analysis using 10620-1-AP

    WB analysis using 10620-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10620-1-AP

    WB analysis using 10620-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.

  • IF Staining of HepG2 using 10620-1-AP

    IF Staining of HepG2 using 10620-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SDHB antibody (10620-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HepG2 using 10620-1-AP

    FC experiment of HepG2 using 10620-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SDHB (10620-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, mouse liver tissue, rat liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10620-1-AP targets SDHB in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Immunogen SDHB fusion protein Ag0968 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Synonyms SDH 1, SDH, PGL4, PGL 4, Iron-sulfur subunit of complex II
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC007840
Gene Symbol SDHB
Gene ID (NCBI) 6390
RRIDAB_2285522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21912
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).

实验方案

Product Specific Protocols
WB protocol for SDHB antibody 10620-1-APDownload protocol
IHC protocol for SDHB antibody 10620-1-APDownload protocol
IF protocol for SDHB antibody 10620-1-APDownload protocol
IP protocol for SDHB antibody 10620-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
  • KD Validated
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mouseWB

Aging Dis

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging

Authors - Minghao Yuan
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humanWB

Cell Death Dis

TGR5 supresses cGAS/STING pathway by inhibiting GRP75-mediated endoplasmic reticulum-mitochondrial coupling in diabetic retinopathy

Authors - Yan Li
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mouseWB

Diabetes

CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis

Authors - Meng Ding
View Article
mouseWB

Free Radic Biol Med

Aristolochic acid I exposure triggers ovarian dysfunction by activating NLRP3 inflammasome and affecting mitochondrial homeostasis

Authors - Ming-Xin Sun
View Article
humanIF

Oxid Med Cell Longev

5-Lipoxygenase Inhibition Protects Retinal Pigment Epithelium from Sodium Iodate-Induced Ferroptosis and Prevents Retinal Degeneration.

Authors - Jong-Jer Lee
View Article