验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue |
Planning an IHC experiment? We recommend our IHCeasy ATP2A2 Ready-To-Use IHC Kit. ATP2A2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
13985-1-AP targets SERCA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SERCA2 fusion protein Ag5052 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 |
| 别名 | ATP2A2, ATP2B, Calcium pump 2, SERCA2, SR Ca(2+) ATPase 2 |
| 计算分子量 | 1042 aa, 115 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC035588 |
| 基因名称 | SERCA2 |
| Gene ID (NCBI) | 488 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P16615 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. SERCA2a and SERCA2c are mainly expressed in cardiac muscle, while SERCA2b is ubiquitously expressed in all cell types. This antibody can recognize all the above isoforms.
发表文章
| Species | Application | Title |
|---|---|---|
Circ Res SPEG Controls Calcium Re-Uptake into the Sarcoplasmic Reticulum Through Regulating SERCA2a by Its Second Kinase-Domain. | ||
Mol Nutr Food Res Sulforaphane Balances Ca2+ Homeostasis Injured by Excessive Fat via Mitochondria-Associated Membrane (MAM). | ||
Toxicol Lett Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo. | ||
J Mol Histol Halofuginone ameliorates the susceptibility to atrial fibrillation by activating the PI3K/Akt signaling pathway |