验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, mouse brain tissue, HEK-293 cells, HeLa cells, mouse lung tissue, K-562 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SF3A1 Ready-To-Use IHC Kit. SF3A1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IP | See 1 publications below |
产品信息
15858-1-AP targets SF3A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SF3A1 fusion protein Ag8196 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | splicing factor 3a, subunit 1, 120kDa |
| 别名 | Spliceosome-associated protein 114, SF3a120, SAP114, SAP 114, PRP21 |
| 计算分子量 | 793 aa, 89 kDa |
| 观测分子量 | 120-130 kDa |
| GenBank蛋白编号 | BC007684 |
| 基因名称 | SF3A1 |
| Gene ID (NCBI) | 10291 |
| RRID | AB_2186354 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15459 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SF3A1 antibody 15858-1-AP | Download protocol |
| IHC protocol for SF3A1 antibody 15858-1-AP | Download protocol |
| IF protocol for SF3A1 antibody 15858-1-AP | Download protocol |
| IP protocol for SF3A1 antibody 15858-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
bioRxiv Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations | ||
Cell Rep Integrative identification of non-coding regulatory regions driving metastatic prostate cancer | ||
Front Pharmacol LXR agonist inhibits inflammation through regulating MyD88 mRNA alternative splicing | ||
bioRxiv Integrative identification of non-coding regulatory regions driving metastatic prostate cancer |
