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验证数据展示

  • WB analysis of NIH/3T3 using 15596-1-AP

    WB analysis of NIH/3T3 using 15596-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15596-1-AP (SF3A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 15596-1-AP

    IHC staining of human endometrial cancer using 15596-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15596-1-AP (SF3A2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human endometrial cancer using 15596-1-AP

    IHC staining of human endometrial cancer using 15596-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15596-1-AP (SF3A2 antibody) at dilution of 1:50 (under 10x lens).

SF3A2 Polyclonal antibody

SF3A2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15596-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (3)

Synonyms

PRP11, PRPF11, SAP 62, SAP62, SF3A2, SF3a66, Splicing factor 3A subunit 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15596-1-AP targets SF3A2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen SF3A2 fusion protein Ag7953 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor 3a, subunit 2, 66kDa
Synonyms PRP11, PRPF11, SAP 62, SAP62, SF3A2, SF3a66, Splicing factor 3A subunit 2
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC004434
Gene Symbol SF3A2
Gene ID (NCBI) 8175
RRIDAB_2301609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15428
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SF3A2 antibody 15596-1-APDownload protocol
IHC protocol for SF3A2 antibody 15596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

J Biol Chem

Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.

Authors - Zhe Chen
View Article
ratWB,IF,CoIP

J Adv Res

Ginsenoside Rb2 inhibits p300-mediated SF3A2 acetylation at lysine 10 to promote Fscn1 alternative splicing against myocardial ischemic/reperfusion injury

Authors - Qingxia Huang
View Article