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  • Featured Product
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SF3A3 Polyclonal antibody

SF3A3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12070-1-AP

Print datasheet

Synonyms

Spliceosome-associated protein 61, SF3a60, SAP61, SAP 61, PRP9

验证数据展示

  • WB analysis using 12070-1-AP

    WB analysis using 12070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12070-1-AP

    IHC staining of human lung cancer using 12070-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12070-1-AP (SF3A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SF3A3 antibody included
  • IP experiment of Raji using 12070-1-AP

    IP experiment of Raji using 12070-1-AP

    IP result of anti-SF3A3 (IP:12070-1-AP, 4ug; Detection:12070-1-AP 1:600) with Raji cells lysate 2000ug.

  • IF Staining of HeLa using 12070-1-AP

    IF Staining of HeLa using 12070-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12070-1-AP (SF3A3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of A549 using 12070-1-AP

    WB analysis of A549 using 12070-1-AP

    WB result of SF3A3 antibody (12070-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SF3A3 transfected A549 cells.

经过测试的应用

Positive WB detected inA431 cells, Raji cells, A549 cells, K-562 cells, NIH/3T3 cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SF3A3 Ready-To-Use IHC Kit. SF3A3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12070-1-AP targets SF3A3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, bovine
Immunogen SF3A3 fusion protein Ag2708 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor 3a, subunit 3, 60kDa
Synonyms Spliceosome-associated protein 61, SF3a60, SAP61, SAP 61, PRP9
Calculated Molecular Weight 501 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC011523
Gene Symbol SF3A3
Gene ID (NCBI) 10946
RRIDAB_2186372
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SF3A3 antibody 12070-1-APDownload protocol
IHC protocol for SF3A3 antibody 12070-1-APDownload protocol
IF protocol for SF3A3 antibody 12070-1-APDownload protocol
IP protocol for SF3A3 antibody 12070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations

Authors - Sihang Zhou
View Article
bovineWB

Int J Mol Sci

Autophagy Mediates Escherichia Coli-Induced Cellular Inflammatory Injury by Regulating Calcium Mobilization, Mitochondrial Dysfunction, and Endoplasmic Reticulum Stress

Authors - Jianguo Liu
View Article