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SHANK1 Polyclonal antibody

SHANK1 Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 55059-1-AP

Print datasheet

Synonyms

SHANK1, SPANK 1, SSTR interacting protein, SSTRIP, synamon

验证数据展示

  • IF Staining of mouse eye using 55059-1-AP

    IF Staining of mouse eye using 55059-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SHANK1 antibody (55059-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse eye using 55059-1-AP

    IF Staining of mouse eye using 55059-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SHANK1 antibody (55059-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 55059-1-AP

    WB analysis using 55059-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55059-1-AP (SHANK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293T using 55059-1-AP

    IP experiment of HEK-293T using 55059-1-AP

    IP result of anti-SHANK1 (IP:55059-1-AP, 4ug; Detection:55059-1-AP 1:400) with HEK-293T cells lysate 1155 ug.

经过测试的应用

Positive WB detected inHEK-293T cells, Neuro-2a cells
Positive IP detected inHEK-293T cells
Positive IF-P detected inmouse eye tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

55059-1-AP targets SHANK1 in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH3 and multiple ankyrin repeat domains 1
Synonyms SHANK1, SPANK 1, SSTR interacting protein, SSTRIP, synamon
Calculated Molecular Weight 225 kDa
Observed Molecular Weight 159-225 kDa
GenBank Accession NumberNM_016148
Gene Symbol SHANK1
Gene ID (NCBI) 50944
RRIDAB_10863130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y566
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHANK1 belongs to the SHANK family. It seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. SHANK1 may play a role in the structural and functional organization of the dendritic spine and synaptic junction. The antibody is specific to SHANK1.

实验方案

Product Specific Protocols
WB protocol for SHANK1 antibody 55059-1-APDownload protocol
IF protocol for SHANK1 antibody 55059-1-APDownload protocol
IP protocol for SHANK1 antibody 55059-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation.

Authors - Zeyu Zhang
View Article
ratWB

Neural Regen Res

Protective effects of Bushen Tiansui decoction on hippocampal synapses in a rat model of Alzheimer's disease.

Authors - Shan Hui
View Article