验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, Jurkat cells, mouse brain tissue, rat testis tissue, U-251 cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human kidney tissue, mouse brain tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17561-1-AP targets SHOC2/Sur-8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, monkey |
| 免疫原 | SHOC2/Sur-8 fusion protein Ag11754 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | soc-2 suppressor of clear homolog (C. elegans) |
| 别名 | SHOC2, Sur-8, Protein sur-8 homolog, Protein sur 8 homolog, Protein soc-2 homolog |
| 计算分子量 | 582 aa, 65 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC050445 |
| 基因名称 | SHOC2/Sur-8 |
| Gene ID (NCBI) | 8036 |
| RRID | AB_2188463 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UQ13 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SHOC2/Sur-8 antibody 17561-1-AP | Download protocol |
| IHC protocol for SHOC2/Sur-8 antibody 17561-1-AP | Download protocol |
| IF protocol for SHOC2/Sur-8 antibody 17561-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Desmoglein-1/Erbin interaction suppresses ERK activation to support epidermal differentiation. | ||
EMBO J Phosphorylation of Pkp1 by RIPK4 regulates epidermal differentiation and skin tumorigenesis. | ||
J Cell Sci Spatial control of Shoc2 scaffold-mediated ERK1/2 signaling requires remodeling activity of the ATPase PSMC5. | ||
Mol Cell Biol HUWE1 Is a Molecular Link Controlling RAF-1 Activity Supported by the Shoc2 Scaffold. | ||
Mol Cancer Res SHOC2 Is a Critical Modulator of Sensitivity to EGFR-TKIs in Non-Small Cell Lung Cancer Cells.
| ||
J Cell Sci The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair.
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