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SHROOM1 Polyclonal antibody

SHROOM1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 18218-1-AP

Print datasheet

Synonyms

Apical protein 2, APXL2, KIAA1960, Protein Shroom1, shroom family member 1, SHROOM1

验证数据展示

  • WB analysis of MCF-7 using 18218-1-AP

    WB analysis of MCF-7 using 18218-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18218-1-AP (SHROOM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 18218-1-AP

    IP experiment of MCF-7 using 18218-1-AP

    IP result of anti-SHROOM1 (IP:18218-1-AP, 4ug; Detection:18218-1-AP 1:700) with MCF-7 cells lysate 3600 ug.

经过测试的应用

Positive WB detected inMCF7 cells, A375 cells
Positive IP detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 18218-1-AP is SHROOM1 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SHROOM1 fusion protein Ag12977 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name shroom family member 1
Synonyms Apical protein 2, APXL2, KIAA1960, Protein Shroom1, shroom family member 1, SHROOM1
Calculated Molecular Weight 852 aa, 91 kDa
Observed Molecular Weight 100-130 kDa
GenBank Accession NumberBC104914
Gene Symbol SHROOM1
Gene ID (NCBI) 134549
RRIDAB_10858616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2M3G4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHROOM1 antibody 18218-1-APDownload protocol
IP protocol for SHROOM1 antibody 18218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Pathog

Defining the proteolytic landscape during enterovirus infection.

Authors - Mohsan Saeed
View Article