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SHROOM1 Polyclonal antibody

SHROOM1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 18218-1-AP

Print datasheet

Synonyms

Apical protein 2, APXL2, KIAA1960, Protein Shroom1, shroom family member 1, SHROOM1

验证数据展示

  • WB analysis of MCF-7 using 18218-1-AP

    WB analysis of MCF-7 using 18218-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18218-1-AP (SHROOM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 18218-1-AP

    IP experiment of MCF-7 using 18218-1-AP

    IP result of anti-SHROOM1 (IP:18218-1-AP, 4ug; Detection:18218-1-AP 1:700) with MCF-7 cells lysate 3600 ug.

经过测试的应用

Positive WB detected inMCF7 cells, A375 cells
Positive IP detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18218-1-AP targets SHROOM1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SHROOM1 fusion protein Ag12977 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name shroom family member 1
Synonyms Apical protein 2, APXL2, KIAA1960, Protein Shroom1, shroom family member 1, SHROOM1
Calculated Molecular Weight 852 aa, 91 kDa
Observed Molecular Weight 100-130 kDa
GenBank Accession NumberBC104914
Gene Symbol SHROOM1
Gene ID (NCBI) 134549
RRIDAB_10858616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2M3G4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHROOM1 antibody 18218-1-APDownload protocol
IP protocol for SHROOM1 antibody 18218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Pathog

Defining the proteolytic landscape during enterovirus infection.

Authors - Mohsan Saeed
View Article