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验证数据展示

  • WB analysis using 60303-1-Ig (same clone as 60303-1-PBS)

    WB analysis using 60303-1-Ig (same clone as 60303-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

  • IF Staining of BT-549 using 60303-1-Ig (same clone as 60303-1-PBS)

    IF Staining of BT-549 using 60303-1-Ig (same clone as 60303-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed BT-549 cells using SIRT1 antibody (60303-1-Ig, Clone: 7F6G6 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

  • WB analysis of Hek-293 cells using 60303-1-Ig (same clone as 60303-1-PBS)

    WB analysis of Hek-293 cells using 60303-1-Ig (same clone as 60303-1-PBS)

    WB result of SIRT1antibody (60303-1-Ig, 1:500) with si-Control and si-SIRT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

  • WB analysis using 60303-1-Ig (same clone as 60303-1-PBS)

    WB analysis using 60303-1-Ig (same clone as 60303-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 60303-1-Ig (same clone as 60303-1-PBS)

    IP experiment of HeLa using 60303-1-Ig (same clone as 60303-1-PBS)

    IP result of anti-SIRT1 (IP:60303-1-Ig, 5ug; Detection:60303-1-Ig 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60303-1-Ig (same clone as 60303-1-PBS)

    IHC staining of human breast cancer using 60303-1-Ig (same clone as 60303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SIRT1 antibody included
  • Featured Product
  • KD/KO验证

SIRT1 Monoclonal antibody, PBS Only

SIRT1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 60303-1-PBS

产品说明书

CloneNo. 7F6G6

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

NAD-dependent protein deacetylase sirtuin-1, hSIRT1, hSIR2, EC:2.3.1.286, EC:2.3.1.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SIRT1 Ready-To-Use IHC Kit. SIRT1 primary antibody included.

产品信息

60303-1-PBS targets SIRT1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 SIRT1 fusion protein Ag17677 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
别名 NAD-dependent protein deacetylase sirtuin-1, hSIRT1, hSIR2, EC:2.3.1.286, EC:2.3.1.-
计算分子量 747 aa, 82 kDa
观测分子量 110-130 kDa
GenBank蛋白编号BC012499
基因名称 SIRT1
Gene ID (NCBI) 23411
RRIDAB_2881417
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96EB6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SIRT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SIRT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.