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  • Featured Product
  • KD/KO Validated

SIRT1 Monoclonal antibody, PBS Only

SIRT1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7F6G6

Cat no : 60303-1-PBS

Print datasheet

Synonyms

NAD-dependent protein deacetylase sirtuin-1, hSIRT1, hSIR2, EC:2.3.1.286, EC:2.3.1.-

验证数据展示

  • WB analysis using 60303-1-Ig (same clone as 60303-1-PBS)

    WB analysis using 60303-1-Ig (same clone as 60303-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

  • IF Staining of BT-549 using 60303-1-Ig (same clone as 60303-1-PBS)

    IF Staining of BT-549 using 60303-1-Ig (same clone as 60303-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed BT-549 cells using SIRT1 antibody (60303-1-Ig, Clone: 7F6G6 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

  • WB analysis of Hek-293 cells using 60303-1-Ig (same clone as 60303-1-PBS)

    WB analysis of Hek-293 cells using 60303-1-Ig (same clone as 60303-1-PBS)

    WB result of SIRT1antibody (60303-1-Ig, 1:500) with si-Control and si-SIRT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

  • WB analysis using 60303-1-Ig (same clone as 60303-1-PBS)

    WB analysis using 60303-1-Ig (same clone as 60303-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 60303-1-Ig (same clone as 60303-1-PBS)

    IP experiment of HeLa using 60303-1-Ig (same clone as 60303-1-PBS)

    IP result of anti-SIRT1 (IP:60303-1-Ig, 5ug; Detection:60303-1-Ig 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60303-1-Ig (same clone as 60303-1-PBS)

    IHC staining of human breast cancer using 60303-1-Ig (same clone as 60303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SIRT1 antibody included
Planning an IHC experiment? We recommend our IHCeasy SIRT1 Ready-To-Use IHC Kit. SIRT1 primary antibody included.

产品信息

60303-1-PBS targets SIRT1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen SIRT1 fusion protein Ag17677 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Synonyms NAD-dependent protein deacetylase sirtuin-1, hSIRT1, hSIR2, EC:2.3.1.286, EC:2.3.1.-
Calculated Molecular Weight 747 aa, 82 kDa
Observed Molecular Weight 110-130 kDa
GenBank Accession NumberBC012499
Gene Symbol SIRT1
Gene ID (NCBI) 23411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96EB6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SIRT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SIRT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.