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验证数据展示

  • WB analysis using 18992-1-AP

    WB analysis using 18992-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa cells using 18992-1-AP

    WB analysis of HeLa cells using 18992-1-AP

    WB result of SLC24A4 antibody (18992-1-AP, 1:1000) with si-Control and si-SLC24A4 transfected HeLa cells.

  • IF Staining of mouse eye using 18992-1-AP

    IF Staining of mouse eye using 18992-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse eye using 18992-1-AP

    IF Staining of mouse eye using 18992-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 18992-1-AP

    IHC staining of mouse brain using 18992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 18992-1-AP

    IHC staining of mouse brain using 18992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 18992-1-AP

    IHC staining of mouse brain using 18992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 18992-1-AP

    IHC staining of mouse brain using 18992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

SLC24A4 Polyclonal antibody

SLC24A4 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 18992-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF-P, ELISA

别名

Solute carrier family 24 member 4, Sodium/potassium/calcium exchanger 4, NCKX4, Na(+)/K(+)/Ca(2+)-exchange protein 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, mouse heart tissue
Positive IHC detected inmouse brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18992-1-AP targets SLC24A4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
别名 Solute carrier family 24 member 4, Sodium/potassium/calcium exchanger 4, NCKX4, Na(+)/K(+)/Ca(2+)-exchange protein 4
计算分子量 69 kDa
观测分子量 62-90 kDa
GenBank蛋白编号NM_153646
基因名称 SLC24A4
Gene ID (NCBI) 123041
RRIDAB_10596939
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NFF2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC24A4, also named as NCKX4, belongs to the sodium/potassium/calcium exchanger family. SLC24A4 transports 1 Ca2+ and 1 K+ in exchange for 4 Na+. Genetic variations in SLC24A4 are associated with skin/hair/eye pigmentation type 6 (SHEP6).

实验方案

Product Specific Protocols
WB protocol for SLC24A4 antibody 18992-1-APDownload protocol
IHC protocol for SLC24A4 antibody 18992-1-APDownload protocol
IF protocol for SLC24A4 antibody 18992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Bone

Deletion of epithelial cell-specific p130Cas impairs the maturation stage of amelogenesis.

Authors - Akane Inoue
View Article
ratWB

J Cell Physiol

Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling.

Authors - Lacruz Rodrigo S RS
View Article
mouseWB

Calcif Tissue Int

Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.

Authors - A L J J Bronckers
View Article
humanWB

PeerJ

Identification and validation of calcium extrusion-related genes prognostic signature in colon adenocarcinoma

Authors - Mingpeng Jin
View Article
mouseWB

Cells Tissues Organs

Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts.

Authors - Hu Ping P
View Article