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CD98/SLC3A2 Polyclonal antibody
CD98/SLC3A2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Raji cells |
| Positive IHC detected in | rat testis tissue, mouse testis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CD98/SLC3A2 Ready-To-Use IHC Kit. CD98/SLC3A2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15193-1-AP targets CD98/SLC3A2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | CD98/SLC3A2 fusion protein Ag7280 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 |
| Synonyms | CD98, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 85-94 kDa, 120-130 kDa |
| GenBank Accession Number | BC001061 |
| Gene Symbol | CD98 |
| Gene ID (NCBI) | 6520 |
| RRID | AB_2254909 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08195 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD98/SLC3A2 antibody 15193-1-AP | Download protocol |
| IHC protocol for CD98/SLC3A2 antibody 15193-1-AP | Download protocol |
| IF protocol for CD98/SLC3A2 antibody 15193-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Nano Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy | ||
Sci Transl Med Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain. | ||
Nat Commun Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters. | ||
J Allergy Clin Immunol Mast cell surfaceome characterization reveals CD98hc is critical for optimal cell function. | ||
J Hazard Mater ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes. | ||
Cell Rep Brown adipocyte ATF4 activation improves thermoregulation and systemic metabolism |
