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Prostein Monoclonal antibody

Prostein Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

5E12E2

Cat no : 60343-1-Ig

Print datasheet

Synonyms

SLC45A3, PRST, Prostate cancer-associated protein 6, PCANAP6, 5E12E2

验证数据展示

  • WB analysis using 60343-1-Ig

    WB analysis using 60343-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 60343-1-Ig

    IHC staining of human prostate cancer using 60343-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60343-1-Ig (Prostein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human prostate cancer using 60343-1-Ig

    IF Staining of human prostate cancer using 60343-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Prostein antibody (60343-1-Ig, Clone: 5E12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inrat testis tissue, DU 145 cells, JAR cells, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60343-1-Ig targets Prostein in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Prostein fusion protein Ag5463 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name solute carrier family 45, member 3
Synonyms SLC45A3, PRST, Prostate cancer-associated protein 6, PCANAP6, 5E12E2
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 55-59 kDa
GenBank Accession NumberBC050416
Gene Symbol Prostein
Gene ID (NCBI) 85414
RRIDAB_2881452
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96JT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Prostein is a prostate tissue-specific protein whose expression is highly restricted to normal and cancerous prostate tissues. Anti-prostein is useful for the identification of prostate adenocarcinomas and is a good marker to identify prostatic origin in metastatic cancer.

实验方案

Product Specific Protocols
WB protocol for Prostein antibody 60343-1-IgDownload protocol
IHC protocol for Prostein antibody 60343-1-IgDownload protocol
IF protocol for Prostein antibody 60343-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols