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验证数据展示

  • WB analysis using 60343-1-Ig

    WB analysis using 60343-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 60343-1-Ig

    IHC staining of human prostate cancer using 60343-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60343-1-Ig (Prostein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human prostate cancer using 60343-1-Ig

    IF Staining of human prostate cancer using 60343-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Prostein antibody (60343-1-Ig, Clone: 5E12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human prostate cancer using 60343-1-Ig

    IHC staining of human prostate cancer using 60343-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60343-1-Ig (Prostein antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Prostein Monoclonal antibody

Prostein Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60343-1-Ig

产品说明书

CloneNo. 5E12E2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

SLC45A3, PRST, Prostate cancer-associated protein 6, PCANAP6, 5E12E2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat testis tissue, DU 145 cells, JAR cells, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60343-1-Ig targets Prostein in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Prostein fusion protein Ag5463 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 45, member 3
别名 SLC45A3, PRST, Prostate cancer-associated protein 6, PCANAP6, 5E12E2
计算分子量 59 kDa
观测分子量 55-59 kDa
GenBank蛋白编号BC050416
基因名称 Prostein
Gene ID (NCBI) 85414
RRIDAB_2881452
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96JT2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Prostein is a prostate tissue-specific protein whose expression is highly restricted to normal and cancerous prostate tissues. Anti-prostein is useful for the identification of prostate adenocarcinomas and is a good marker to identify prostatic origin in metastatic cancer.

实验方案

Product Specific Protocols
WB protocol for Prostein antibody 60343-1-IgDownload protocol
IHC protocol for Prostein antibody 60343-1-IgDownload protocol
IF protocol for Prostein antibody 60343-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols