- Featured Product
- KD/KO Validated
SMAD3 Polyclonal antibody
SMAD3 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (2)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, A431 cells, A549 cells, HeLa cells, SH-SY5Y cells |
Positive IHC detected in | human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25494-1-AP targets SMAD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Applications | WB, IF, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse, rat, canine, bovine |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | SMAD family member 3 |
Synonyms | hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3 |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 48 kDa |
GenBank Accession Number | NM_005902 |
Gene Symbol | SMAD3 |
Gene ID (NCBI) | 4088 |
RRID | AB_2880102 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P84022 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SMAD3 antibody 25494-1-AP | Download protocol |
IHC protocol for SMAD3 antibody 25494-1-AP | Download protocol |
IF protocol for SMAD3 antibody 25494-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Circulation Proefferocytic Therapy Promotes Transforming Growth Factor-β Signaling and Prevents Aneurysm Formation. | ||
Cell Death Differ REGγ ablation impedes dedifferentiation of anaplastic thyroid carcinoma and accentuates radio-therapeutic response by regulating the Smad7-TGF-β pathway. | ||
Cell Mol Life Sci SGK3 deficiency in macrophages suppresses angiotensin II-induced cardiac remodeling via regulating Ndufa13-mediated mitochondrial oxidative stress | ||
Oncogene The β-catenin/TCF-4-LINC01278-miR-1258-Smad2/3 axis promotes hepatocellular carcinoma metastasis.
| ||
Stem Cell Reports Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.
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Oxid Med Cell Longev WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling. |